A Golgi-Located Transmembrane Nine Protein Gene TMN11 Functions in Manganese/Cadmium Homeostasis and Regulates Growth and Seed Development in Rice
Abstract
:1. Introduction
2. Results
2.1. Structure and Sequence of OsTMN11 Gene and Protein
2.2. OsTMN11 Is Localized to the Cis-Golgi Apparatus and Induced under Mn and Cd Stress
2.3. OsTMN11 Knockdown RNAi Lines Are Sensitive to Excessive Mn and Cd Ions
2.4. OsTMN11 Knockdown Results in Overaccumulation of Mn and Cd in Rice Plants
2.5. OsTMN11 Can Complement a Mn-Sensitive Phenotype of Golgi-Resident pmr1 Mutant Yeast and Regulate Cellular Mn Efflux
2.6. OsTMN11 Suppression Dampens Rice Growth and Development under Natural Conditions
2.7. OsTMN11 Disruption Impaired Mn and Cd Accumulation in Maturity Rice Plants
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Materials and Treatment
5.2. Analysis of Bioinformatics Related to OsTMN11 Gene and Protein Sequences
5.3. Transcriptional Expression of OsTMN11
5.4. Generation of Rice RNAi Lines
5.5. Analysis of Subcellular Localization of OsTMN11
5.6. Transformation OsTMN11 into Yeast Cells
5.7. Measurement of Metal Concentration
5.8. Measurement of Chlorophyll and Malondialdehyde
5.9. Analysis of Morphology and Phenotypes Relevant to Seed Development
5.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, H.; Li, C.; Liu, X.; Yang, Z. A Golgi-Located Transmembrane Nine Protein Gene TMN11 Functions in Manganese/Cadmium Homeostasis and Regulates Growth and Seed Development in Rice. Int. J. Mol. Sci. 2022, 23, 15883. https://doi.org/10.3390/ijms232415883
Li H, Li C, Liu X, Yang Z. A Golgi-Located Transmembrane Nine Protein Gene TMN11 Functions in Manganese/Cadmium Homeostasis and Regulates Growth and Seed Development in Rice. International Journal of Molecular Sciences. 2022; 23(24):15883. https://doi.org/10.3390/ijms232415883
Chicago/Turabian StyleLi, He, Chao Li, Xuesong Liu, and Zhimin Yang. 2022. "A Golgi-Located Transmembrane Nine Protein Gene TMN11 Functions in Manganese/Cadmium Homeostasis and Regulates Growth and Seed Development in Rice" International Journal of Molecular Sciences 23, no. 24: 15883. https://doi.org/10.3390/ijms232415883