Synergistic Effects of Ginsenoside Rb3 and Ferruginol in Ischemia-Induced Myocardial Infarction
Abstract
:1. Introduction
2. Results
2.1. The Combination of G-Rb3 and FGL Improved Cardiac Function and Protected Cardiac Structure in LAD-Induced MI Mice
2.2. The Combination of G-Rb3 and FGL Alleviated Dyslipidemia and Oxidative Stress in LAD-Induced MI Mice
2.3. G-Rb3-FGL Protected H9C2 Cells against OGD/R-Induced Cellular Injury
2.4. Effects of G-Rb3-FGL Co-Treatment on Fatty Acid Oxidization in OGD/R-Stimulated H9C2 Cells
2.5. Effects of G-Rb3-FGL Co-Treatment on Oxidative Stress and Inflammation Response in OGD/R-Stimulated H9C2 Cells
2.6. G-Rb3-FGL Inhibited Apoptosis via RXRα In Vitro
2.7. FGL Had the Potential to Improve the Oral Bioavailability of G-Rb3
3. Discussion
4. Materials and Methods
4.1. MI Model Establishment and Animal Grouping
4.2. Cardiac Functions and Histological Assessment
4.3. Serum Detection
4.4. Cell Culture
4.5. Intracellular ROS Detection
4.6. ATP and Mitochondrial Membrane Potential Assessment
4.7. Detection of Intracellular Rh123 Content
4.8. Western Blot Analysis
Antibodies | Source | Identifier |
anti-RXRα antibody | Abcam, Cambrige, UK | Cat#ab125001 |
anti-CD36 antibody | Abcam, Cambrige, UK | Cat#ab252922 |
anti-CPT1A antibody | Abcam, Cambrige, UK | Cat#ab128568 |
anti-ACADL antibody | Abcam, Cambrige, UK | Cat#ab196655 |
anti-p65-NF-κB antibody | Abways, Shanghai, China | Cat#CY5034 |
anti-TNF-α antibody | Abcam, Cambrige, UK | Cat#ab307164 |
anti-IL-1β antibody | ProteinTech Group, Rosemont, IL, USA | Cat#10663 |
anti-COX2 antibody | Abcam, Cambrige, UK | Cat#ab179800 |
anti-Bax antibody | Abcam, Cambrige, UK | Cat#ab32503 |
anti-Bcl-2 antibody | Abcam, Cambrige, UK | Cat#ab196495 |
anti-HO-1 antibody | Abways, Shanghai, China | Cat#CY5113 |
anti-Nrf-2 antibody | Abways, Shanghai, China | Cat#CY5136 |
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chen, X.; Liu, T.; Wang, Q.; Wang, H.; Xue, S.; Jiang, Q.; Li, J.; Li, C.; Wang, W.; Wang, Y. Synergistic Effects of Ginsenoside Rb3 and Ferruginol in Ischemia-Induced Myocardial Infarction. Int. J. Mol. Sci. 2022, 23, 15935. https://doi.org/10.3390/ijms232415935
Chen X, Liu T, Wang Q, Wang H, Xue S, Jiang Q, Li J, Li C, Wang W, Wang Y. Synergistic Effects of Ginsenoside Rb3 and Ferruginol in Ischemia-Induced Myocardial Infarction. International Journal of Molecular Sciences. 2022; 23(24):15935. https://doi.org/10.3390/ijms232415935
Chicago/Turabian StyleChen, Xu, Tiantian Liu, Qiyan Wang, Hui Wang, Siming Xue, Qianqian Jiang, Junjun Li, Chun Li, Wei Wang, and Yong Wang. 2022. "Synergistic Effects of Ginsenoside Rb3 and Ferruginol in Ischemia-Induced Myocardial Infarction" International Journal of Molecular Sciences 23, no. 24: 15935. https://doi.org/10.3390/ijms232415935