An ABCG-Type Transporter Facilitates ABA Influx and Regulates Camptothecin Biosynthesis in Camptotheca acuminata
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of CaABAT
2.2. Functional Identification of CaABAT in Yeast Cells
2.3. Expression Analysis and Subcellular Localization of CaABAT
2.4. Exogenous Plant Hormone Treatments on C. acuminata Seedlings
2.5. Silencing and Overexpression of CaABAT in C. acuminata
3. Discussion
3.1. CaABAT Belongs to the ABCG Subfamily and Is Involved in ABA Transport
3.2. Effect of CaABAT on ABA-Regulated Camptothecin Biosynthesis
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Isolation and Characterization of CaABAT
4.3. RNA Extraction and Real-Time PCR Analysis
4.4. Transient Overexpression and Virus-Induced Gene Silencing (VIGS) of CaABAT in C. acuminata
4.5. Functional Identification of CaABAT in Yeast Cells
4.6. Subcellular Localization of CaABAT
4.7. Exogenous Plant Hormone Treatment
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Y.; Wang, Y.; Bai, H.; Han, Y.; Yu, F. An ABCG-Type Transporter Facilitates ABA Influx and Regulates Camptothecin Biosynthesis in Camptotheca acuminata. Int. J. Mol. Sci. 2022, 23, 16120. https://doi.org/10.3390/ijms232416120
Wang Y, Wang Y, Bai H, Han Y, Yu F. An ABCG-Type Transporter Facilitates ABA Influx and Regulates Camptothecin Biosynthesis in Camptotheca acuminata. International Journal of Molecular Sciences. 2022; 23(24):16120. https://doi.org/10.3390/ijms232416120
Chicago/Turabian StyleWang, Yanyan, Yang Wang, Hefei Bai, Yuqian Han, and Fang Yu. 2022. "An ABCG-Type Transporter Facilitates ABA Influx and Regulates Camptothecin Biosynthesis in Camptotheca acuminata" International Journal of Molecular Sciences 23, no. 24: 16120. https://doi.org/10.3390/ijms232416120