Molecular Mechanisms Underlying Metabolic Resistance to Cyflumetofen and Bifenthrin in Tetranychus urticae Koch on Cowpea
Abstract
:1. Introduction
2. Results
2.1. Selection of the Cyflumetofen- and Bifenthrin-Resistant Strains
2.2. The Enzyme Activity in the Cyflumetofen- and Bifenthrin-Resistant Strains
2.3. Transcriptome Sequencing, Data Processing and Differential Gene Expression Analysis
2.4. Functional Annotation
2.4.1. Functional Annotation of the Lab_SS vs R_cfm Group
2.4.2. Functional Annotation of the Lab_SS vs R_bft Group
2.4.3. Functional Annotation of the Common DEGs between the Lab_SS vs R_cfm Group and the Lab_SS vs R_bft Group
2.5. Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analysis
2.5.1. KEGG Pathway Analysis of the Lab_SS vs R_cfm Group
2.5.2. KEGG Pathway Analysis of the Lab_SS vs R_bft Group
2.5.3. KEGG Pathway Analysis of the Common DEGs between the Lab_SS vs R_cfm Group and the Lab_SS vs R_bft Group
2.6. Selected DEGs of Detoxification Enzymes of Lab_SS vs R_cfm and Lab_SS vs R_bft
2.7. Validation of Detoxification Enzyme Genes
3. Discussion
4. Materials and Methods
4.1. Mites
4.2. Reagents
4.3. Resistance Selection
4.4. Bioassays of Cyflumetofen- and Bifenthrin-Resistant Strains of T. urticae
4.5. Determination of the Activity of the Detoxification Enzymes UGTs, GSTs, CarEs and P450s
4.6. Transcriptome Sequencing
4.6.1. Total RNA Extraction, Library Construction and RNA-Seq
4.6.2. Transcriptome Sequencing Data Analysis
4.6.3. Transcriptome Sequencing Data Analysis
4.7. Quantitative Real-Time PCR (qRT-PCR) Analysis
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Acaricide | Generation | Regression | LC50 (mg/L) (95% CI) | Resistance Ratio |
---|---|---|---|---|
Cyflumetofen | F0 | y = 4.14 + 1.10x | 6.02 (4.29~8.43) | - |
F16 | y = −6.65 + 4.09x | 707.95 (646.64~775.09) | 117.60 | |
Bifenthrin | F0 | y = −0.21 + 2.46x | 125.04 (103.19~151.52) | - |
F20 | - | >20000 | >159.95 |
Samples | Total Raw Reads (Mb) | Total Clean Reads (Mb) | Clean Reads Ratio (%) | Mapping Rate (%) | Clean Reads Q20 (%) | Clean Reads Q30 (%) |
---|---|---|---|---|---|---|
Lab_SS1 | 45.57 | 42.24 | 92.68 | 63.45 | 94.94 | 88.86 |
Lab_SS2 | 47.33 | 42.83 | 90.50 | 62.02 | 94.55 | 88.10 |
Lab_SS3 | 45.57 | 42.26 | 92.72 | 64.88 | 94.67 | 88.30 |
R_cfm1 | 47.33 | 43.11 | 91.10 | 67.04 | 94.72 | 88.42 |
R_cfm2 | 42.65 | 38.51 | 90.30 | 70.27 | 94.80 | 88.53 |
R_cfm3 | 47.33 | 43.10 | 91.07 | 66.80 | 94.84 | 88.66 |
R_bft1 | 49.08 | 42.81 | 87.22 | 49.29 | 95.17 | 89.34 |
R_bft2 | 47.33 | 43.10 | 91.08 | 54.89 | 94.85 | 88.69 |
R_bft3 | 45.57 | 42.22 | 92.65 | 69.80 | 94.70 | 88.36 |
Detoxification Enzymes | Gene ID | log2 (R_cfm or R_bft /Lab_SS) | Symbol | |
---|---|---|---|---|
Lab_SS vs R_cfm | CCEs | tetur17g00350 | 1.77 | TuCCE46 |
tetur207g00010 | 1.28 | TuCCE70 | ||
P450s | tetur07g06440 | 1.64 | CYP392A2p | |
tetur07g06410 | 1.10 | CYP392A1 | ||
tetur27g00340 | −1.32 | CYP392E7 | ||
Lab_SS vs R_bft | CCEs | tetur17g00350 | 3.68 | TuCCE46 |
tetur207g00010 | 3.09 | TuCCE70 | ||
tetur207g00020 | 2.65 | CCEincTu16 | ||
tetur17g00300 | 2.63 | TuCCE45 | ||
tetur17g00080 | 2.21 | TuCCE44 | ||
tetur17g00360 | 2.04 | CCEincTu08 | ||
tetur35g00180 | 1.89 | CCEincTu13 | ||
tetur35g00200 | 1.77 | TuCCE65 | ||
tetur04g06770 | 1.53 | TuCCE25 | ||
tetur01g14180 | 1.26 | TuCCE12 | ||
tetur11g05770 | 1.15 | TuCCE35 | ||
tetur16g02380 | −1.01 | TuCCE40 | ||
tetur04g02550 | −1.04 | TuCCE22 | ||
tetur30g01290 | −1.26 | TuCCE61 | ||
tetur01g08680 | −1.46 | TuCCE01 | ||
UGTs | tetur04g07630 | 3.31 | UGT16 | |
tetur05g00080 | 2.83 | UGT22 | ||
tetur21g01400 | 2.29 | UGT58p | ||
tetur22g00420 | 1.75 | UGT65 | ||
tetur08g00190 | 1.73 | UGT40 | ||
tetur09g01660 | 1.69 | UGT | ||
tetur05g09325 | 1.56 | UGT | ||
tetur05g00090 | 1.30 | UGT23 | ||
tetur22g00510 | 1.17 | UGT69 | ||
P450s | tetur03g04990 | 2.04 | CYP392D2 | |
tetur03g00970 | 1.75 | CYP392A11 | ||
tetur03g09961 | −1.14 | CYP392D7 | ||
tetur46g00150 | −1.44 | CYP385C4v2 | ||
tetur07g06440 | −1.89 | CYP392A2p | ||
GSTs | tetur31g01330 | 1.88 | TuGSTd15 | |
tetur05g05260 | 1.65 | TuGSTm09 | ||
tetur26g01510 | 1.49 | TuGSTd13 | ||
tetur05g05250 | 1.48 | TuGSTm08 | ||
ABCs | tetur09g01970 | 1.35 | TuABCG-13 | |
tetur18g00230 | 1.14 | TuABCH-13 | ||
tetur11g02120 | 1.06 | TuABCC-28 |
Gene | Forward Primer | Reverse Primer |
---|---|---|
tetur03g04990 | TCTAAAGGACCAAGAGGAG | ACGATGGGTTTGATAATGT |
tetur04g07630 | ATCAGGACCTCCACCATTT | AATCTATTCGGCACCAACC |
tetur05g00080 | AACATCGCTTCATCGTCTC | CAATCAATCTTGCCACTTC |
tetur21g01400 | GGACCAAGAGGAGACGAAC | TTCAGCATACGATGGGTTT |
tetur17g00350 | TTCCTTATGCGAAGCCAACG | AACGGACCAAGATCCAGCA |
tetur207g00010 | AAAAGGACCAGCGAAGACT | AACGGACCAAGATCCAGCA |
tetur207g00020 | TATGTTTTCGGTTTACCTC | TTTCCCCAATCATCTATG |
tetur17g00300 | CAAGAAGAACCAGCGAAGA | CTGACCAAGATCCAGCAGA |
tetur17g00080 | AACATCGCTTCATCGTCTC | CAATCAATCTTGCCACTTC |
tetur17g00360 | TCAAACAAACTACGACCAG | CTCCGAAGTAGATGAAACC |
tetur01g08680 | TTTTGATAATTCGGTTGACG | ACGATATTGGCTTAGGAGG |
tetur22g00420 | ATGGTCCACCTCTTCGTTC | TTCCCATTGAGACATAGATAAGC |
tetur03g00970 | AGGAAGCAATTCGCTGATA | GCCAACAATAGGAAGACCC |
tetur07g06440 | CTCGTCCAATATCCTCAAT | AGCACTAAATGGCACTAAA |
tetur31g01330 | GATTGGTTTACGCTGTCCT | GTTTCGTTTCGATAAGATTG |
tetur05g05260 | AGGTGCTGTTGAGCCTATT | TTTCTTGGTCCTAAATCGT |
tetur09g01970 | ATGTTGCTGGCTGAGGGTC | ATGGCTTCTTGATTGTAGTCCTG |
tetur27g00340 | GGCTTAGGAAAGAGTGAAA | CGAAATACGGAAACAGACC |
tetur01g12670 | CTTCAAGGCGGTGACTTTACC | CCATTGAAAGGATACCTGGTCC |
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Liu, Z.; Wu, F.; Liang, W.; Zhou, L.; Huang, J. Molecular Mechanisms Underlying Metabolic Resistance to Cyflumetofen and Bifenthrin in Tetranychus urticae Koch on Cowpea. Int. J. Mol. Sci. 2022, 23, 16220. https://doi.org/10.3390/ijms232416220
Liu Z, Wu F, Liang W, Zhou L, Huang J. Molecular Mechanisms Underlying Metabolic Resistance to Cyflumetofen and Bifenthrin in Tetranychus urticae Koch on Cowpea. International Journal of Molecular Sciences. 2022; 23(24):16220. https://doi.org/10.3390/ijms232416220
Chicago/Turabian StyleLiu, Zhenxiu, Fuxing Wu, Weikang Liang, Lijuan Zhou, and Jiguang Huang. 2022. "Molecular Mechanisms Underlying Metabolic Resistance to Cyflumetofen and Bifenthrin in Tetranychus urticae Koch on Cowpea" International Journal of Molecular Sciences 23, no. 24: 16220. https://doi.org/10.3390/ijms232416220