Transcription Factor RrANT1 of Rosa rugosa Positively Regulates Flower Organ Size in Petunia hybrida
Abstract
:1. Introduction
2. Results
2.1. Identification and Phylogenetic Analysis of RrAP2 Family
2.2. RrANT1 Identification and Characterization
2.3. Relative Expression Level of RrANT1 in the Flower Tissues and Leaves of R. rugosa
2.4. Subcellular Localization of RrANT1
2.5. Phenotypic and Microstructure Observation of Leaves and Flowers of Transgenic Petunia
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification, Phylogenetic, Gene Structure, and Motif Composition Analysis of AP2 Family
4.3. The Extraction of Nucleic Acid
4.4. Cloning of Full-Length cDNA of RrANT1
4.5. Reverse Transcription Reaction and RrANT1 Expression Analysis
4.6. Vector Construction and Subcellular Localization of RrANT1
4.7. Overexpression (OE) of RrANT1 in P. hybrida and Microstructural Observation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xu, Y.; Xing, Y.; Wei, T.; Wang, P.; Liang, Y.; Xu, M.; Ding, H.; Wang, J.; Feng, L. Transcription Factor RrANT1 of Rosa rugosa Positively Regulates Flower Organ Size in Petunia hybrida. Int. J. Mol. Sci. 2022, 23, 1236. https://doi.org/10.3390/ijms23031236
Xu Y, Xing Y, Wei T, Wang P, Liang Y, Xu M, Ding H, Wang J, Feng L. Transcription Factor RrANT1 of Rosa rugosa Positively Regulates Flower Organ Size in Petunia hybrida. International Journal of Molecular Sciences. 2022; 23(3):1236. https://doi.org/10.3390/ijms23031236
Chicago/Turabian StyleXu, Yong, Yongxiang Xing, Tiantian Wei, Pengqing Wang, Yue Liang, Mengmeng Xu, Haiquan Ding, Jianwen Wang, and Liguo Feng. 2022. "Transcription Factor RrANT1 of Rosa rugosa Positively Regulates Flower Organ Size in Petunia hybrida" International Journal of Molecular Sciences 23, no. 3: 1236. https://doi.org/10.3390/ijms23031236