The Effect of RADA16-I and CDNF on Neurogenesis and Neuroprotection in Brain Ischemia-Reperfusion Injury
Abstract
:1. Introduction
2. Results
2.1. CDNF, but Not RADA16-I, Promotes the Proliferation of Cultured NSCs
2.2. RADA16-I, but Not CDNF, Promotes the Neural Differentiation of Cultured NSCs
2.3. RADA16-I and CDNF Promote the Proliferation of NSCs and Neuroblasts in the SVZ of Ischemia/Reperfusion Rats
2.4. CDNF, but Not RADA16-I, Promotes the Migration of Neuroblasts in the SVZ into the Ischemic Penumbra Area of Ischemia/Reperfusion Rats
2.5. RADA16-I and CDNF Promote Neural Differentiation in Ischemia/Reperfusion Rats
2.6. Pathways Involved in the Neurogenesis of CDNF and RADA16-I in Ischemia/Reperfusion Rats
2.7. CDNF, but Not RADA16-I, Has a Neuroprotective Effect on Ischemia/Reperfusion Rats
3. Discussion
4. Materials and Methods
4.1. Experimental Materials
4.2. NSC Culture
4.3. Immunocytochemistry
4.4. MCAO Model
4.5. Intracerebroventricular Microinjection
4.6. BrdU Labeling
4.7. Immunohistochemistry
4.8. Evaluation of Infarct Volume
4.9. Examination of Neurological Deficits
4.10. Western Blotting
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
Abbreviations
CNS | central nervous system |
DAPI | 4,6-diamidino-2-phenylindole dihydrochloride hydrate |
CDNF | cerebral dopamine neurotrophic factor |
NSCs | neural stem cells |
LV | lateral ventricle |
MCAO | middle cerebral artery occlusion |
NTFs | neurotrophic factors |
NGF | nerve growth factor |
BDNF | brain-derived neurotrophic factor |
GDNF | glial cell line-derived neurotrophic factor |
OGD | oxygen-glucose deprivation |
SVZ | subventricular zone |
SGZ | subgranular zone |
DG | dentate gyrus |
MANF | mesencephalic astrocyte-derived neurotrophic factor |
GFAP | glial fibrillary acidic protein |
CCA | common carotid artery |
ECA | external carotid artery |
ICA | internal carotid artery |
MCA | middle cerebral artery |
DCX | doublecortin |
TTC | triphenyltetrazolium chloride |
SEM | standard error of the mean |
HRP | horseradish peroxidase |
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Liu, X.; Ren, H.; Peng, A.; Cheng, H.; Chen, J.; Xia, X.; Liu, T.; Wang, X. The Effect of RADA16-I and CDNF on Neurogenesis and Neuroprotection in Brain Ischemia-Reperfusion Injury. Int. J. Mol. Sci. 2022, 23, 1436. https://doi.org/10.3390/ijms23031436
Liu X, Ren H, Peng A, Cheng H, Chen J, Xia X, Liu T, Wang X. The Effect of RADA16-I and CDNF on Neurogenesis and Neuroprotection in Brain Ischemia-Reperfusion Injury. International Journal of Molecular Sciences. 2022; 23(3):1436. https://doi.org/10.3390/ijms23031436
Chicago/Turabian StyleLiu, Xingyu, Haiyuan Ren, Ai Peng, Haoyang Cheng, Jiahao Chen, Xue Xia, Ting Liu, and Xiaojing Wang. 2022. "The Effect of RADA16-I and CDNF on Neurogenesis and Neuroprotection in Brain Ischemia-Reperfusion Injury" International Journal of Molecular Sciences 23, no. 3: 1436. https://doi.org/10.3390/ijms23031436