Role of Autophagy in Lysophosphatidylcholine-Induced Apoptosis of Mouse Ovarian Granulosa Cells
Abstract
:1. Introduction
2. Results
2.1. LPC Induces Apoptosis of Mouse Ovarian Granulosa Cells
2.2. LPC Induces Autophagy of Mouse Ovarian Granulosa Cells
2.3. LPC Induces Oxidative Stress in Mouse Ovarian Granulosa Cells
2.4. Oxidative Stress is Involved in LPC-Induced Apoptosis
2.5. Oxidative Stress Is Involved in LPC-Induced Autophagy
2.6. Autophagy Affects LPC-Induced Apoptosis in Mouse Ovarian Granulosa Cells
3. Discussion
4. Materialsand Methods
4.1. Materials and Reagents
4.2. Isolation of Mouse Ovarian Granulosa Cells
4.3. The FSHR Fluorescence Staining
4.4. Cell Counting Kti-8(CCK-8) Assay
4.5. Western Blotting Analysis
4.6. Oxidative Stress Measurement
4.7. AnnexinV-FITC/PI Apoptosis Assay
4.8. Transmission Electron Microscopy Analysis
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, S.; Chen, J.; Ma, B.; Wang, J.; Chen, J. Role of Autophagy in Lysophosphatidylcholine-Induced Apoptosis of Mouse Ovarian Granulosa Cells. Int. J. Mol. Sci. 2022, 23, 1479. https://doi.org/10.3390/ijms23031479
Yang S, Chen J, Ma B, Wang J, Chen J. Role of Autophagy in Lysophosphatidylcholine-Induced Apoptosis of Mouse Ovarian Granulosa Cells. International Journal of Molecular Sciences. 2022; 23(3):1479. https://doi.org/10.3390/ijms23031479
Chicago/Turabian StyleYang, Si, Jie Chen, Bingchun Ma, Jinglei Wang, and Jiaxiang Chen. 2022. "Role of Autophagy in Lysophosphatidylcholine-Induced Apoptosis of Mouse Ovarian Granulosa Cells" International Journal of Molecular Sciences 23, no. 3: 1479. https://doi.org/10.3390/ijms23031479