Transcriptional Regulation of Yin-Yang 1 Expression through the Hypoxia Inducible Factor-1 in Pediatric Acute Lymphoblastic Leukemia
Abstract
:1. Introduction
2. Results
2.1. Transcriptional Regulation of the YY1 Protein by HIF-1α in Leukemia Cell Lines
2.2. Induction or Inhibition of HIF-1α Expression Affects in the YY1 Expression
2.3. Correlation of HIF-1α and YY1 Expression in Pediatric Patients with ALL
2.4. Network Analysis of HIF-1α/YY1 and Correlation between HIF-1α and YY1 Expression in ALL
3. Discussion
4. Materials and Methods
4.1. Ethical and Biosecurity Aspects
4.2. Patients
4.3. Cell Culture
4.4. Determination of the Putative Binding Sites of HIF1α in the YY1 Promoter
4.5. Cloning of the Promoter Region of YY1
4.6. Site-Direct Mutagenesis in Putative Binding Sites for HIF-1α of the Promoter YY1
4.7. Transfection of Cell Lines with the Construct Generated
4.8. Chromatin Immunoprecipitation
4.9. Treatments and Exposure of ALL Cells to Hypoxic Conditions
4.10. Immunocytochemistry
4.11. Real-Time RT-PCR Assays
4.12. Cell Viability Assays
4.13. Network Analysis of HIF-1α/YY1 and the Construction of Gene Networks Related to Function
4.14. Bioinformatics Analysis of and Correlation between HIF-1α and YY1 Gene Expression in ALL
4.15. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Total Number | 108 |
---|---|
Gender | |
Female | 45 |
Male | 63 |
Age (years) | 7.8 (0.1–16) |
Phenotype | |
B | 32 |
Pre-B | 40 |
Pro-B | 13 |
T | 11 |
Unknown | 12 |
Survivor | 79 |
Deaths | 29 |
Name | Sequence |
---|---|
YY1pro_Sen (BglI underlined) | CGAGATCTGCTTTTTTGAACAGAGAGCC |
YY1Pro_Ant (BamH1 underlined) | GAGGATCCGGGTGCAAACCG |
YY1mutAant (Mutation in bold) | GCCCGCGGCGAAGAACGTCAGCGCGCCGCCGCC |
YY1mutAsen (Mutation in bold) | GGCGGCGGCGCGCTGACGTTCTTCGCCGCGGGC |
YY1mutBant (Mutation in bold) | GGCGGGGCGGCTCGAGAACGCCCTGGCTGGCC |
YY1mutBsen (Mutation in bold) | GGCCAGCCAGGGCGTTCTCGAGCCGCCCCGCC |
YY1mutCant (Mutation in bold) | GTGGTGGTGGCCGGAAGACCCGTGGCCGCCCC |
YY1mutCsen (Mutation in bold) | GGGGCGGCCACGGGTCTTCCGGCCACCACCAC |
Ch(PromYY)_HIFABsen | TTTTGTGGCTGTTGCACCG |
Ch(PromYY)_HIFABant | AATCGATCTGTCCGCTGGC |
Ch(PromYY)_HIFCsen | AGACCATCGAGACCACAGTGGTGG |
Ch(PromYY)_HIFant | CTGCAGAGCGATCATGGGCG |
Ch(PromTel)_HIFposSen | AGCGCTGCGTCCTGCT |
Ch(PromTel)_HIFposAnt | AGCACCTCGCGGTAGTGG |
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Antonio-Andres, G.; Martinez-Ruiz, G.U.; Morales-Martinez, M.; Jiménez-Hernandez, E.; Martinez-Torres, E.; Lopez-Perez, T.V.; Estrada-Abreo, L.A.; Patino-Lopez, G.; Juarez-Mendez, S.; Davila-Borja, V.M.; et al. Transcriptional Regulation of Yin-Yang 1 Expression through the Hypoxia Inducible Factor-1 in Pediatric Acute Lymphoblastic Leukemia. Int. J. Mol. Sci. 2022, 23, 1728. https://doi.org/10.3390/ijms23031728
Antonio-Andres G, Martinez-Ruiz GU, Morales-Martinez M, Jiménez-Hernandez E, Martinez-Torres E, Lopez-Perez TV, Estrada-Abreo LA, Patino-Lopez G, Juarez-Mendez S, Davila-Borja VM, et al. Transcriptional Regulation of Yin-Yang 1 Expression through the Hypoxia Inducible Factor-1 in Pediatric Acute Lymphoblastic Leukemia. International Journal of Molecular Sciences. 2022; 23(3):1728. https://doi.org/10.3390/ijms23031728
Chicago/Turabian StyleAntonio-Andres, Gabriela, Gustavo U. Martinez-Ruiz, Mario Morales-Martinez, Elva Jiménez-Hernandez, Estefany Martinez-Torres, Tania V. Lopez-Perez, Laura A. Estrada-Abreo, Genaro Patino-Lopez, Sergio Juarez-Mendez, Víctor M. Davila-Borja, and et al. 2022. "Transcriptional Regulation of Yin-Yang 1 Expression through the Hypoxia Inducible Factor-1 in Pediatric Acute Lymphoblastic Leukemia" International Journal of Molecular Sciences 23, no. 3: 1728. https://doi.org/10.3390/ijms23031728