Expression and Function of Nicotinic Acetylcholine Receptors in Induced Regulatory T Cells
Abstract
:1. Introduction
2. Results
2.1. Effect of Nicotine on iTreg Cells
2.2. Expression of nAChRs on T Cell Subsets
2.3. Mechanisms Regulating nAChR9 Expression in iTreg Cells
3. Discussion
4. Materials and Methods
4.1. T-Cell Subset Preparation
4.2. Cytokine Production and Proliferation of iTreg Cells
4.3. Expression of nAChRs
4.4. Chromatin Immunoprecipitation (ChIP) Assay
4.5. Reporter Assay
4.6. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Nakata, Y.; Miura, K.; Yamasaki, N.; Ogata, S.; Miura, S.; Hosomi, N.; Kaminuma, O. Expression and Function of Nicotinic Acetylcholine Receptors in Induced Regulatory T Cells. Int. J. Mol. Sci. 2022, 23, 1779. https://doi.org/10.3390/ijms23031779
Nakata Y, Miura K, Yamasaki N, Ogata S, Miura S, Hosomi N, Kaminuma O. Expression and Function of Nicotinic Acetylcholine Receptors in Induced Regulatory T Cells. International Journal of Molecular Sciences. 2022; 23(3):1779. https://doi.org/10.3390/ijms23031779
Chicago/Turabian StyleNakata, Yuichiro, Kento Miura, Norimasa Yamasaki, Sawako Ogata, Shuka Miura, Naohisa Hosomi, and Osamu Kaminuma. 2022. "Expression and Function of Nicotinic Acetylcholine Receptors in Induced Regulatory T Cells" International Journal of Molecular Sciences 23, no. 3: 1779. https://doi.org/10.3390/ijms23031779