Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R
Abstract
:1. Introduction
2. Results
2.1. Sequence Analysis of maIL-6 and masIL-6R
2.2. Effects of Recombinant IL-6 on the Expression of Downstream Genes in L8824 Cells
2.3. Activation of Signaling Pathways by rmaIL-6 with or without RmasIL-6R
2.4. RmaIL-6 Trans-Signaling Regulates Socs3a and Socs3b Expression via the JAK2/STAT3 Pathway in L8824 Cells and CIK Cells
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Fish
4.2. Isolation and Culture of Hepatocytes
4.3. Sequence Obtainment and Analysis
4.4. RNA Extraction and cDNA Synthesis
4.5. Expression and Purification of the Recombinant Proteins ciIL-6, maIL-6, and masIL-6R
4.6. Treatment of Cells
4.7. qPCR Analysis
4.8. Protein Extraction and Quantification
4.9. Western Blot
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
IL-6 | Interleukin-6 |
IL-6R | Interleukin-6 receptor |
IL-1β | Interleukin-1β |
Hamp | Hepcidin |
SOCS3 | Suppressor of cytokine signaling 3 |
ciIL-6 | Grass carp IL-6 |
ciIL-6R | Grass carp IL-6R |
maIL-6 | Blunt snout bream IL-6 |
maIL-6R | Blunt snout bream IL-6R |
masIL-6R | Blunt snout bream soluble IL-6R receptor |
rciIL-6 | Recombinant grass carp IL-6 protein |
rmaIL-6 | Recombinant blunt snout bream IL-6 protein |
rmasIL-6R | Recombinant blunt snout bream soluble IL-6 receptor protein |
JAK | Janus kinases |
STAT | Signal transducer and activator of transcription |
gp130 | Glycoprotein 130 |
ERK | Extracellular-signal-regulated kinase |
MEK | Mitogen-activated protein kinase |
PI3K | Phosphatidylinositol 3 kinase |
AKT | Protein kinase b |
mIL-6R | Membrane-bound IL-6R |
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Wang, J.; Sun, Q.; Zhang, J.; Wang, H.; Liu, H. Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R. Int. J. Mol. Sci. 2022, 23, 2019. https://doi.org/10.3390/ijms23042019
Wang J, Sun Q, Zhang J, Wang H, Liu H. Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R. International Journal of Molecular Sciences. 2022; 23(4):2019. https://doi.org/10.3390/ijms23042019
Chicago/Turabian StyleWang, Jixiu, Qianhui Sun, Jian Zhang, Huanling Wang, and Hong Liu. 2022. "Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R" International Journal of Molecular Sciences 23, no. 4: 2019. https://doi.org/10.3390/ijms23042019