The Daily Expression of ABCC4 at the BCSFB Affects the Transport of Its Substrate Methotrexate
Abstract
:1. Introduction
2. Results
2.1. Sex-Dependent Daily Oscillations of Membrane Transporters in Rat Choroid Plexus
2.2. hAbcc4 and hAbcg2 mRNA Circadian Expression in the HIBCPP Cell Line
2.3. Circadian Oscillations in MTX Transport across BCSFB
2.4. Effects of ABCC4 and ABCG2 Inhibition in the MTX Circadian Transport across BCSFB
3. Discussion
4. Material and Methods
4.1. Animals and Cell Line
4.2. HIBCPP Cell Culture
4.3. Quantitative Real-Time PCR (qPCR)
4.4. MTX Uptake Assay
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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rAbcc1 | Intact males | p-value = 0.0086 COG = 4.65 |
rAbcc4 | Intact females | p-value = 0.0080 COG = 14.20 |
Intact males | p-value = 0.0429 COG = 7.43 | |
rAbcg2 | Intact females | p-value = 0.0447 COG = 13.66 |
rOat3 | Intact females | p-value = 0.0079 COG = 17.27 |
Intact males | p-value = 0.0312 COG = 18.80 |
hAbcc4 | p-value = 0.0068 COG = 9.91 |
hAbcg2 | p-value = 0.0001 COG = 10.74 |
Gene | Primer Sequence (5′–3′) | Amplicon Size (bp) |
---|---|---|
hAbcc4 | FW: TGTGGCTTTGAACACAGCGTA | 105 |
RV: CCAGCACACTGAACGTGATAA | ||
hAbcg2 | FW: ACGAACGGATTAACAGGGTCA | 93 |
RV: CTCCAGACACACCACGGAT | ||
hGapdh * | FW: ATGGGGAAGGTGAAGGTCG | 108 |
RV: GGGGTCATTGATGGCAACAATA | ||
rAbca1 | FW: CGGCGGAGTAGAAAGGGTTT | 84 |
RV: CACGATCAGGCTGAAGACCAG | ||
rAbcb1 | FW: AAGGGGCTACAGGGTCTAGG | 100 |
RV: AGTGTCAATTGCCAGCCGTA | ||
rAbcc1 | FW: TTCATATCTGCTTCGTCACCG | 60 |
RV: CGTAAACAGCACCCACCACAGC | ||
rAbcc4 | FW: TTCCCCTTCGACCTTATCCT | 124 |
RV: TAGGCAGCTGTTGTCAGTGG | ||
rAbcg2 | FW: GGCCTGGACAAAGTAGCAGA | 137 |
RV: GTTGTGGGCTCATCCAGGAA | ||
rAbcg4 | FW: ATGGCTGATGTACCCTTCCAGGTT | 155 |
RV: ATCAAGAGTCCCAAAGACTGGGCA | ||
rOat3 | FW: GAGGACCTGTGATTGGAGAACTG | 82 |
RV: CTG GCT GCC AGC ATG AGA TA | ||
rCycA * | FW: CAAGACTGAGTGGCTGGATGG | 163 |
RV: GCCCGCAAGTCAAAGAAATTAGAG |
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Furtado, A.; Mineiro, R.; Duarte, A.C.; Gonçalves, I.; Santos, C.R.; Quintela, T. The Daily Expression of ABCC4 at the BCSFB Affects the Transport of Its Substrate Methotrexate. Int. J. Mol. Sci. 2022, 23, 2443. https://doi.org/10.3390/ijms23052443
Furtado A, Mineiro R, Duarte AC, Gonçalves I, Santos CR, Quintela T. The Daily Expression of ABCC4 at the BCSFB Affects the Transport of Its Substrate Methotrexate. International Journal of Molecular Sciences. 2022; 23(5):2443. https://doi.org/10.3390/ijms23052443
Chicago/Turabian StyleFurtado, André, Rafael Mineiro, Ana Catarina Duarte, Isabel Gonçalves, Cecília R. Santos, and Telma Quintela. 2022. "The Daily Expression of ABCC4 at the BCSFB Affects the Transport of Its Substrate Methotrexate" International Journal of Molecular Sciences 23, no. 5: 2443. https://doi.org/10.3390/ijms23052443
APA StyleFurtado, A., Mineiro, R., Duarte, A. C., Gonçalves, I., Santos, C. R., & Quintela, T. (2022). The Daily Expression of ABCC4 at the BCSFB Affects the Transport of Its Substrate Methotrexate. International Journal of Molecular Sciences, 23(5), 2443. https://doi.org/10.3390/ijms23052443