Dek504 Encodes a Mitochondrion-Targeted E+-Type Pentatricopeptide Repeat Protein Essential for RNA Editing and Seed Development in Maize
Abstract
:1. Introduction
2. Results
2.1. Genetic and Phenotypic Characterization of the dek504-ref Mutant
2.2. Positional Cloning of dek504
2.3. Dek504 Encodes a Mitochondrion-Targeted E+-Type PPR Protein
2.4. DEK504 Is Required for the C-to-U Editing of Mitochondrial nad3 Transcript
2.5. Dek504 Mutation Affects Mitochondrial Complex I Assembly and NADH Dehydrogenase Activity
2.6. Mitochondria Morphology Was Affected and an Alternative Respiratory Pathway Was Activated in the dek504-ref Mutant
3. Discussion
3.1. DEK504 Encodes an E+-Type PPR Protein Involved in Editing at nad3-44 and nad3-317
3.2. Dek504 Editing Sites Are Important for Mitochondrial Function
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Light Microscopy of Cytological Sections, TEM, and SEM
4.3. Protein and Starch Content Measurement
4.4. Map-Based dek504 Cloning
4.5. 3′-RACE
4.6. Subcellular Localization
4.7. RNA Extraction, RT-PCR, and qRT-PCR
4.8. Mitochondrial RNA Editing Analysis
4.9. Isolation and Analysis of Mitochondria Complexes and Western Blotting
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Z.; Chen, W.; Zhang, S.; Lu, J.; Chen, R.; Fu, J.; Gu, R.; Wang, G.; Wang, J.; Cui, Y. Dek504 Encodes a Mitochondrion-Targeted E+-Type Pentatricopeptide Repeat Protein Essential for RNA Editing and Seed Development in Maize. Int. J. Mol. Sci. 2022, 23, 2513. https://doi.org/10.3390/ijms23052513
Wang Z, Chen W, Zhang S, Lu J, Chen R, Fu J, Gu R, Wang G, Wang J, Cui Y. Dek504 Encodes a Mitochondrion-Targeted E+-Type Pentatricopeptide Repeat Protein Essential for RNA Editing and Seed Development in Maize. International Journal of Molecular Sciences. 2022; 23(5):2513. https://doi.org/10.3390/ijms23052513
Chicago/Turabian StyleWang, Zheyuan, Weiwei Chen, Song Zhang, Jiawen Lu, Rongrong Chen, Junjie Fu, Riliang Gu, Guoying Wang, Jianhua Wang, and Yu Cui. 2022. "Dek504 Encodes a Mitochondrion-Targeted E+-Type Pentatricopeptide Repeat Protein Essential for RNA Editing and Seed Development in Maize" International Journal of Molecular Sciences 23, no. 5: 2513. https://doi.org/10.3390/ijms23052513