Gene-Delivery Ability of New Hydrogenated and Partially Fluorinated Gemini bispyridinium Surfactants with Six Methylene Spacers
Abstract
:1. Introduction
2. Results
2.1. Synthesis
2.2. EMSA and MTT Assays
2.3. AFM and DLS
2.4. Transient Transfection Experiments
3. Materials and Methods
3.1. Compounds
3.1.1. Chemical Synthesis
Synthesis of the 1,6-Bis(2-pyridyl)hexane
Synthesis of 1,1′-Bis(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl)-2,2′-hexamethylenebis (Pyridinium) Chloride
Synthesis of 1,1′-Bis-hexadecyl-2,2′-hexamethylenebispyridinium Chloride (GP16_6)
3.2. DNA Preparation and Storage
3.3. Cell Culture
3.4. Electrophoresis Mobility Shift Assay (EMSA)
3.5. MTT Proliferation Assay
3.6. Transient Transfection Assay
3.7. Sample Preparation and Atomic Force Microscopy (AFM) Imaging
3.8. Dynamic Light Scattering (DLS)
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Massa, M.; Rivara, M.; Donofrio, G.; Cristofolini, L.; Peracchia, E.; Compari, C.; Bacciottini, F.; Orsi, D.; Franceschi, V.; Fisicaro, E. Gene-Delivery Ability of New Hydrogenated and Partially Fluorinated Gemini bispyridinium Surfactants with Six Methylene Spacers. Int. J. Mol. Sci. 2022, 23, 3062. https://doi.org/10.3390/ijms23063062
Massa M, Rivara M, Donofrio G, Cristofolini L, Peracchia E, Compari C, Bacciottini F, Orsi D, Franceschi V, Fisicaro E. Gene-Delivery Ability of New Hydrogenated and Partially Fluorinated Gemini bispyridinium Surfactants with Six Methylene Spacers. International Journal of Molecular Sciences. 2022; 23(6):3062. https://doi.org/10.3390/ijms23063062
Chicago/Turabian StyleMassa, Michele, Mirko Rivara, Gaetano Donofrio, Luigi Cristofolini, Erica Peracchia, Carlotta Compari, Franco Bacciottini, Davide Orsi, Valentina Franceschi, and Emilia Fisicaro. 2022. "Gene-Delivery Ability of New Hydrogenated and Partially Fluorinated Gemini bispyridinium Surfactants with Six Methylene Spacers" International Journal of Molecular Sciences 23, no. 6: 3062. https://doi.org/10.3390/ijms23063062