cDNA Transcriptome of Arabidopsis Reveals Various Defense Priming Induced by a Broad-Spectrum Biocontrol Agent Burkholderia sp. SSG

Round 1

Reviewer 1 Report

The manuscript "cDNA transcriptome of Arabidopsis reveals various defense 2 priming induced by a broad-spectrum biocontrol agent 3 Burkholderia sp. SSG" is well written, covering all the relevant aspects of the topic.

There are minor writing errors such as use consistent units (for time use one of h or hr or hour). Use one unit throughout. Minor sentencing errors that should be easily fixed in re-reading of manuscript.

I am not sure, if any submissions to NCBI database are needed for this manuscript. Kindly look into it.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

Dear Authors

The current manuscript entitled “cDNA transcriptome of Arabidopsis reveals various defense  priming induced by a broad-spectrum biocontrol agent Burkholderia sp. SSG” demonstrated and suggested that SSG is an induced systemic resistance (ISR) trigger conferring plant protection upon pathogen encounter. The present work has been very well planned, executed and presented nicely. Although there are some queries and further opportunities to improve the manuscript.

1. For cell suspension preparation, a dish of the subculture was suspended in 100 ml Phosphate-Buffered Saline (PBS) to have a concentration at 10⁸ to 10⁹ CFU mL-1. Please explain if colony from subculture was grown in PBS, or the scraped colonies were just mixed in PBS? And the concentration was 10⁸ or 10⁹?
2. Humidity in the growth chamber during the experiment?
3. A small concluding remark may be included to highlight the key finding of the study.

Author Response

Please see the attachment.

Author Response File: Author Response.docx