TaKLU Plays as a Time Regulator of Leaf Growth via Auxin Signaling
Abstract
:1. Introduction
2. Results
2.1. Overexpression of TaKLU Increased Leaf Size and Biomass by Promoting Cell Division
2.2. TaKLU Overexpression Extended the Time of Leaf Elongation
2.3. Overexpression of TaKLU Smoothed the Fluctuations of Leaf Initiation Time in Wheat
2.4. TaKLU Was Functionally Conserved in Both Monocots and Dicots
2.5. TaKLU Promoted Leaf Growth via Auxin Signaling
3. Discussion
3.1. KLU Plays as a Time Regulator of Leaf Growth
3.2. Relationship between KLU and Plant Hormones
3.3. Strategies of TaKLU Applied in Wheat Breeding
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Vector Construction and Transformation
4.3. RNA Isolation and QRT-PCR Analysis
4.4. Germination Experiment
4.5. Morphological Analysis
4.6. Chemical Treatments
4.7. Cytological Analysis
4.8. Histochemical Staining
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhou, M.; Peng, H.; Wu, L.; Li, M.; Guo, L.; Chen, H.; Wu, B.; Liu, X.; Zhao, H.; Li, W.; et al. TaKLU Plays as a Time Regulator of Leaf Growth via Auxin Signaling. Int. J. Mol. Sci. 2022, 23, 4219. https://doi.org/10.3390/ijms23084219
Zhou M, Peng H, Wu L, Li M, Guo L, Chen H, Wu B, Liu X, Zhao H, Li W, et al. TaKLU Plays as a Time Regulator of Leaf Growth via Auxin Signaling. International Journal of Molecular Sciences. 2022; 23(8):4219. https://doi.org/10.3390/ijms23084219
Chicago/Turabian StyleZhou, Mengdie, Haixia Peng, Linnan Wu, Mengyao Li, Lijian Guo, Haichao Chen, Baowei Wu, Xiangli Liu, Huixian Zhao, Wenqiang Li, and et al. 2022. "TaKLU Plays as a Time Regulator of Leaf Growth via Auxin Signaling" International Journal of Molecular Sciences 23, no. 8: 4219. https://doi.org/10.3390/ijms23084219