Overexpression of Nucleolin and Associated Genes in Prostate Cancer
Abstract
:1. Introduction
2. Results
2.1. Baseline Patient Characteristics
2.2. Nucleolin Is Overexpressed in Prostate Cancer
2.3. NCL Expression Is Not a Prognosis Biomarker of PCa
2.4. Genes Positively Correlated with NCL Have Function in Cell Division and Ribosome Biogenesis
2.5. Tumor Cell Lines Highly Expressed NCL
3. Discussion
4. Materials and Methods
4.1. Human Prostate Cancer Specimens
4.2. RNA Microarray and Transcriptomic Data
4.3. Bioinformatic Analysis
4.4. Human Prostate Cancer Tissues and Immunohistochemistry
4.5. Cell Culture
4.6. Western Blot
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | Mean/Median (Range) | No. of Patients (%) |
---|---|---|
Age, y | 62.87/63 (44–76) | |
PSA, ng/mL | 11.16/7 (1.7–76) | |
Follow-up n = 113, mo | 99.3/105.7 (1.4–173.9) | |
Pathological tumor classification | ||
pT2a/b | 9 (7.7%) | |
pT2c | 28 (24.1%) | |
pT3a | 54 (46.5%) | |
pT3b | 19 (16.3%) | |
pT4 | 6 (5.1%) | |
Prostatectomy gleason score | ||
6 | 20 (17.2%) | |
7 (3 + 4) | 25 (21.5%) | |
7 (4 + 3) | 40 (34.5%) | |
8 | 26 (22.4%) | |
9 | 5 (4.3%) | |
Relapse n = 113 | ||
yes | 56 | |
no | 57 |
TCGA | PAIR Prostate | GIANT | ||||
---|---|---|---|---|---|---|
Correlated Gene | Cytoband | Spearman’s Correlation | p-Value | Spearman’s Correlation | p-Value | Score |
HSPD1 | 2q33.1 | 0.6890 | 5.62 x10-70 | 0.6844 | <0.0001 | 0.668 |
PAICS | 4q12 | 0.6780 | 5.62 x10-67 | 0.5860 | <0.0001 | 0.595 |
CCT5 | 5p15.2 | 0.6327 | 5.88 x10-56 | 0.5402 | <0.0001 | 0.558 |
NPM1 | 5q35.1 | 0.6087 | 8.06 x10-51 | 0.4385 | <0.0001 | 0.657 |
SERBP1 | 1p31.3 | 0.5227 | 1.40 x10-35 | 0.5038 | <0.0001 | 0.691 |
GART | 21q22.11 | 0.5145 | 2.45 x10--34 | 0.5501 | <0.0001 | 0.534 |
PA2G4 | 12q13.2 | 0.5054 | 5.20 x10--33 | 0.5700 | <0.0001 | 0.708 |
PAICS | 4q12 | 0.6780 | 5.62 x10--67 | 0.5860 | <0.0001 | 0.595 |
CCT5 | 5p15.2 | 0.6327 | 5.88 x10--56 | 0.5402 | <0.0001 | 0.558 |
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Firlej, V.; Soyeux, P.; Nourieh, M.; Huet, E.; Semprez, F.; Allory, Y.; Londono-Vallejo, A.; de la Taille, A.; Vacherot, F.; Destouches, D. Overexpression of Nucleolin and Associated Genes in Prostate Cancer. Int. J. Mol. Sci. 2022, 23, 4491. https://doi.org/10.3390/ijms23094491
Firlej V, Soyeux P, Nourieh M, Huet E, Semprez F, Allory Y, Londono-Vallejo A, de la Taille A, Vacherot F, Destouches D. Overexpression of Nucleolin and Associated Genes in Prostate Cancer. International Journal of Molecular Sciences. 2022; 23(9):4491. https://doi.org/10.3390/ijms23094491
Chicago/Turabian StyleFirlej, Virginie, Pascale Soyeux, Maya Nourieh, Eric Huet, Fannie Semprez, Yves Allory, Arturo Londono-Vallejo, Alexandre de la Taille, Francis Vacherot, and Damien Destouches. 2022. "Overexpression of Nucleolin and Associated Genes in Prostate Cancer" International Journal of Molecular Sciences 23, no. 9: 4491. https://doi.org/10.3390/ijms23094491