Next Article in Journal
Streptozotocin-Induced Diabetes Causes Changes in Serotonin-Positive Neurons in the Small Intestine in Pig Model
Next Article in Special Issue
Novel Antibody–Peptide Binding Assay Indicates Presence of Immunoglobulins against EGFR Phospho-Site S1166 in High-Grade Glioma
Previous Article in Journal
Ac/Ds-Induced Receptor-like Kinase Genes Deletion Provides Broad-Spectrum Resistance to Bacterial Blight in Rice
Previous Article in Special Issue
GZ17-6.02 Inhibits the Growth of EGFRvIII+ Glioblastoma
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 9
10.3390/ijms23094562
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Crosstalk between β-Catenin and CCL2 Drives Migration of Monocytes towards Glioblastoma Cells

by
Philippe Aretz
1,
Donata Maciaczyk
2,
Suad Yusuf
1,
Rüdiger V. Sorg
3,
Daniel Hänggi
1,
Hongjia Liu
4,
Hongde Liu
4,
Tikam Chand Dakal
5,
Amit Sharma
6,
Ramakrishna Bethanabatla
7,
Silke Neumann
2 and
Jarek Maciaczyk
6,8,*
1
Department of Neurosurgery, University Hospital Düsseldorf, 40225 Dusseldorf, Germany
2
Department of Pathology, University of Otago, Dunedin 9016, New Zealand
3
Institute for Transplantation Diagnostics and Cell Therapeutics, University Hospital Düsseldorf, 40225 Dusseldorf, Germany
4
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China
5
Genome and Computational Biology Lab, Department of Biotechnology, Mohanlal Sukhadia University, Udaipur 313001, India
6
Department of Stereotactic and Functional Neurosurgery, University Hospital Bonn, 53127 Bonn, Germany
7
Northumbria Healthcare NHS Foundation Trust, Newcastle upon Tyne NE27 0QJ, UK
8
Department of Surgical Sciences, University of Otago, Dunedin 9016, New Zealand
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(9), 4562; https://doi.org/10.3390/ijms23094562
Submission received: 14 March 2022 / Revised: 13 April 2022 / Accepted: 14 April 2022 / Published: 20 April 2022
(This article belongs to the Special Issue Glioblastoma: Recapitulating the Key Breakthroughs and Future Perspective)
Browse Figures
Versions Notes

Abstract

Isocitrate dehydrogenase (IDH)-wildtype glioblastoma (GBM) is a fast growing and highly heterogeneous tumor, often characterized by the presence of glioblastoma stem cells (GSCs). The plasticity of GSCs results in therapy resistance and impairs anti-tumor immune response by influencing immune cells in the tumor microenvironment (TME). Previously, β-catenin was associated with stemness in GBM as well as with immune escape mechanisms. Here, we investigated the effect of β-catenin on attracting monocytes towards GBM cells. In addition, we evaluated whether CCL2 is involved in β-catenin crosstalk between monocytes and tumor cells. Our analysis revealed that shRNA targeting β-catenin in GBMs reduces monocytes attraction and impacts CCL2 secretion. The addition of recombinant CCL2 restores peripheral blood mononuclear cells (PBMC) migration towards medium (TCM) conditioned by shβ-catenin GBM cells. CCL2 knockdown in GBM cells shows similar effects and reduces monocyte migration to a similar extent as β-catenin knockdown. When investigating the effect of CCL2 on β-catenin activity, we found that CCL2 modulates components of the Wnt/β-catenin pathway and alters the clonogenicity of GBM cells. In addition, the pharmacological β-catenin inhibitor MSAB reduces active β-catenin, downregulates the expression of associated genes and alters CCL2 secretion. Taken together, we showed that β-catenin plays an important role in attracting monocytes towards GBM cells in vitro. We hypothesize that the interactions between β-catenin and CCL2 contribute to maintenance of GSCs via modulating immune cell interaction and promoting GBM growth and recurrence.
Keywords: glioblastoma; GSCs; β-catenin; Wnt; CCL2; monocytes; immune evasion; MSAB glioblastoma; GSCs; β-catenin; Wnt; CCL2; monocytes; immune evasion; MSAB

Share and Cite

MDPI and ACS Style

Aretz, P.; Maciaczyk, D.; Yusuf, S.; Sorg, R.V.; Hänggi, D.; Liu, H.; Liu, H.; Dakal, T.C.; Sharma, A.; Bethanabatla, R.; et al. Crosstalk between β-Catenin and CCL2 Drives Migration of Monocytes towards Glioblastoma Cells. Int. J. Mol. Sci. 2022, 23, 4562. https://doi.org/10.3390/ijms23094562

AMA Style

Aretz P, Maciaczyk D, Yusuf S, Sorg RV, Hänggi D, Liu H, Liu H, Dakal TC, Sharma A, Bethanabatla R, et al. Crosstalk between β-Catenin and CCL2 Drives Migration of Monocytes towards Glioblastoma Cells. International Journal of Molecular Sciences. 2022; 23(9):4562. https://doi.org/10.3390/ijms23094562

Chicago/Turabian Style

Aretz, Philippe, Donata Maciaczyk, Suad Yusuf, Rüdiger V. Sorg, Daniel Hänggi, Hongjia Liu, Hongde Liu, Tikam Chand Dakal, Amit Sharma, Ramakrishna Bethanabatla, and et al. 2022. "Crosstalk between β-Catenin and CCL2 Drives Migration of Monocytes towards Glioblastoma Cells" International Journal of Molecular Sciences 23, no. 9: 4562. https://doi.org/10.3390/ijms23094562

APA Style

Aretz, P., Maciaczyk, D., Yusuf, S., Sorg, R. V., Hänggi, D., Liu, H., Liu, H., Dakal, T. C., Sharma, A., Bethanabatla, R., Neumann, S., & Maciaczyk, J. (2022). Crosstalk between β-Catenin and CCL2 Drives Migration of Monocytes towards Glioblastoma Cells. International Journal of Molecular Sciences, 23(9), 4562. https://doi.org/10.3390/ijms23094562

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop