Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins
Abstract
:1. Introduction
2. Results
2.1. Structure and Sequence Analysis of the Matrix Salt Bridge Network of MCs
2.2. Effects on Activity by Mutating the Charged Residue Positions in the PX[DE]XX[KR] Motifs of OGC
2.3. Effects on Activity by Mutating the Charged Residue Positions in the PX[DE]XX[KR] Motifs of Ggc1p
2.4. Effects on Activity by Mutating the Charged Residue Positions in the PX[DE]XX[KR] Motifs of Ndt1p
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Construction of Plasmids and Site-Directed Mutagenesis
4.3. Overexpression and Purification of the Recombinant MC Proteins
4.4. Reconstitution of the Purified MC Proteins into Liposomes and Transport Measurements
4.5. Additional Experimental Methods
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Miniero, D.V.; Monné, M.; Di Noia, M.A.; Palmieri, L.; Palmieri, F. Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins. Int. J. Mol. Sci. 2022, 23, 5060. https://doi.org/10.3390/ijms23095060
Miniero DV, Monné M, Di Noia MA, Palmieri L, Palmieri F. Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins. International Journal of Molecular Sciences. 2022; 23(9):5060. https://doi.org/10.3390/ijms23095060
Chicago/Turabian StyleMiniero, Daniela Valeria, Magnus Monné, Maria Antonietta Di Noia, Luigi Palmieri, and Ferdinando Palmieri. 2022. "Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins" International Journal of Molecular Sciences 23, no. 9: 5060. https://doi.org/10.3390/ijms23095060