Next Article in Journal
Focused Design of Novel Cyclic Peptides Endowed with GABARAP-Inhibiting Activity
Previous Article in Journal
Overexpression of EgrIAA20 from Eucalyptus grandis, a Non-Canonical Aux/IAA Gene, Specifically Decouples Lignification of the Different Cell-Types in Arabidopsis Secondary Xylem
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 23
Issue 9
10.3390/ijms23095065
Review Report
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation

Int. J. Mol. Sci. 2022, 23(9), 5065; https://doi.org/10.3390/ijms23095065
by Constance Mayslich 1, Philippe Alain Grange 1,2,3, Mathieu Castela 1, Anne Geneviève Marcelin 4, Vincent Calvez 4 and Nicolas Dupin 1,2,3,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2022, 23(9), 5065; https://doi.org/10.3390/ijms23095065
Submission received: 15 April 2022 / Revised: 30 April 2022 / Accepted: 1 May 2022 / Published: 3 May 2022
(This article belongs to the Topic Toll-Like Receptors and Their Potential Roles in Human Diseases)

Round 1

Reviewer 1 Report

In the presented manuscript authors identified and described CAMP1 peptide as a TLR-2 modulator. The study includes considerable number of assays. Results are well presented and conclusion is supported by the evidence.

Language is fine - minor text required.

Minor questions: why absorbance of MTT was evaluated at 540 nm?

Author Response

In the presented manuscript authors identified and described CAMP1 peptide as a TLR-2 modulator. The study includes considerable number of assays. Results are well presented and conclusion is supported by the evidence.

Language is fine - minor text required.

Minor questions: why absorbance of MTT was evaluated at 540 nm?

Answer:

  • The manuscript has been carefully read
  • The MTT was measured at 550 nm, we corrected the value in the Material & Method section in lane 840

Reviewer 2 Report

I really liked this paper and found it scientifically sound.  I do not that in the first paragraph, you mention C. acnes is anerobic, but you should add that is aerotolerant as well (as this can be significant clinically).  

I also would like you to expand on your conclusion as to how you believe a TLR-2 modulator might be functional in acne therapy.  

Author Response

I really liked this paper and found it scientifically sound.

I do not that in the first paragraph, you mention C. acnes is anerobic, but you should add that is aerotolerant as well (as this can be significant clinically).  

I also would like you to expand on your conclusion as to how you believe a TLR-2 modulator might be functional in acne therapy.  

Answer:

  • We agreed that C. acnes is able to growth under normal atmosphere, consequently we added the aerotolerant term in lane 35.
  • We added a sentence in the conclusion about the test of this peptide on a acne patients:

“C. acnes is able to trigger inflammation reaction through the TLR-2 pathway and TLR-2 is highly expressed in acne lesion. Therefore, this candidate peptide appears to be a new TLR-2 modulator which could be clinically tested on patients presenting inflammatory acne lesions.”

Back to TopTop