Mechanisms of Resistance to Spot Blotch in Yunnan Iron Shell Wheat Based on Metabolome and Transcriptomics
Abstract
:1. Introduction
2. Results
2.1. Detection Results and Analysis of Widely Targeted Metabolome
2.1.1. Quality Control of Widely Targeted Metabolome Data
2.1.2. Qualitative and Quantitative Analysis of Metabolome
2.1.3. Differential Analysis of Metabolic Profiles between Resistant and Susceptible Strains
2.1.4. Enrichment Analysis of Differential Metabolite KEGG Pathway after SB Infection
2.2. Transcriptome Test Results and Analysis
2.2.1. Assembly Notes
2.2.2. Differential Genes and GO and KEGG Enrichment Analysis
2.2.3. Screening of Genes Related to Metabolic Pathway Differences
2.2.4. RT-qPCR Validation
2.3. Combined Analysis of Transcription and Metabolome
2.3.1. Connection between DEGs and Dams
2.3.2. Correlation Analysis of Differential Genes and Metabolites in “Benzoxazinoid Biosynthesis” and “Arginine and Proline Metabolism”
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Widely Targeted Metabolome Detection and Analysis
4.3. Transcriptome Sequencing and Analysis
4.4. Combined Analysis of Transcriptome and Metabolome
4.5. RT-qPCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species of Metabolites | Number of Metabolites |
---|---|
amino acids and their derivatives | 95 |
phenolic acids | 178 |
nucleotides and their derivatives | 67 |
flavone | 234 |
lignans and coumarins | 30 |
blending quality | 3 |
alkaloid | 111 |
terpenoids | 22 |
organic acid | 84 |
lipid | 154 |
other | 100 |
total: | 1078 |
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Zhang, X.; Huang, T.; Wang, Q.; Guo, Y.; Zhang, P.; Xie, H.; Liu, J.; Li, L.; Zhang, C.; Qin, P. Mechanisms of Resistance to Spot Blotch in Yunnan Iron Shell Wheat Based on Metabolome and Transcriptomics. Int. J. Mol. Sci. 2022, 23, 5184. https://doi.org/10.3390/ijms23095184
Zhang X, Huang T, Wang Q, Guo Y, Zhang P, Xie H, Liu J, Li L, Zhang C, Qin P. Mechanisms of Resistance to Spot Blotch in Yunnan Iron Shell Wheat Based on Metabolome and Transcriptomics. International Journal of Molecular Sciences. 2022; 23(9):5184. https://doi.org/10.3390/ijms23095184
Chicago/Turabian StyleZhang, Xuesong, Tingzhi Huang, Qianchao Wang, Yirui Guo, Ping Zhang, Heng Xie, Junna Liu, Li Li, Chuanli Zhang, and Peng Qin. 2022. "Mechanisms of Resistance to Spot Blotch in Yunnan Iron Shell Wheat Based on Metabolome and Transcriptomics" International Journal of Molecular Sciences 23, no. 9: 5184. https://doi.org/10.3390/ijms23095184