The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum
Abstract
:1. Introduction
2. Results
2.1. Identification of miRNAs Expressed in the CNS of Helix
2.2. miRNAs Expression Dynamics in the Helix CNS after Learning
2.3. miRNAs Expression in the CNS of Learning Deficient DHT-Treated Helix after Training
3. Discussion
4. Materials and Methods
4.1. Animals and Conditioned Reflex Formation
4.2. Drugs and the Injection Procedure
4.3. RNA Isolation and Sequencing
4.4. Bioinformatics Analysis
4.5. cDNA Synthesis and Real-Time RT-PCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Subfamily | Seed Region | Helix miRNA | Human Homologue |
---|---|---|---|
MIR-10-P1 (mir-10) | ACCCUGU | from hlu-mir-10-S1 to hlu-mir-10-S3 | hsa-mir-10a [Gene ID: 406902] |
MIR-10-P2 (mir-99/100) | ACCCGUA | from hlu-mir-10-S4 to hlu-mir-10-S7 | hsa-mir-100 [Gene ID: 406892] |
MIR-10-P3 (mir-125) | CCCUGAG | from hlu-mir-10-S8 to hlu-mir-10-S26 | hsa-mir-125b [Gene ID: 406911] |
MicroRNA dynamics 2 h after training | |||||
MicroRNA | Normal animals | DHT treated | MicroRNA | Normal animals | DHT treated |
Hlu-Mir-2-S1_3p | ⇩ | Hlu-Mir-76-S3_3p | ↑ | ||
Hlu-Mir-10-S5_5p | ↑ | Hlu-Mir-133_3p | ↑ | ||
Hlu-Mir-10-S6_5p | ⇩ | Hlu-Mir-193_3p | ↑ | ||
Hlu-Mir-10-S12_5p | ⇩ | Hlu-Mir-277_3p | ↑ | ||
Hlu-Mir-10-S18_5p | ⇩ | Hlu-Mir-278_3p | ↑ | ||
Hlu-Mir-10-S19_5p | ⇩ | Hlu-Mir-1985-S2_3p | ↑ | ||
Hlu-Mir-33_5p | ↑ | Hlu-Mir-2722-S1_3p | ⇩ | ||
MicroRNA dynamics 5 h after training | |||||
MicroRNA | Normal animals | DHT treated | MicroRNA | Normal animals | DHT treated |
Hlu-Let-7-S1_5p | ↑ | Hlu-Mir-71_3p | ⇩ | ⇩ | |
Hlu-Mir-2-S1_3p | ⇩ | Hlu-Mir-87_3p | ⇩ | ⇩ | |
Hlu-Mir-7_5p | ⇩ | Hlu-Mir-92-S1_3p | ↑ | ||
Hlu-Mir-8_3p | ⇩ | ⇩ | Hlu-Mir-92-S2_3p | ⇩ | ⇩ |
Hlu-Mir-9_5p | ⇩ | Hlu-Mir-92-S3_3p | ⇩ | ⇩ | |
Hlu-Mir-10-S8_5p | ↑ | ↑ | Hlu-Mir-96_5p | ↑ | |
Hlu-Mir-10-S9_5p | ↑ | ↑ | Hlu-Mir-124_3p | ⇩ | ⇩ |
Hlu-Mir-10-S10_5p | ↑ | ↑ | Hlu-Mir-133_3p | ⇩ | ⇩ |
Hlu-Mir-10-S14_5p | ↑ | ↑ | Hlu-Mir-137_3p | ⇩ | ⇩ |
Hlu-Mir-10-S15_5p | ⇩ | ⇩ | Hlu-Mir-153-S1_3p | ⇩ | |
Hlu-Mir-10-S17_5p | ↑ | ↑ | Hlu-Mir-153-S2_3p | ⇩ | |
Hlu-Mir-10-S20_5p | ↑ | ↑ | Hlu-Mir-210_3p | ⇩ | ⇩ |
Hlu-Mir-10-S21_5p | ↑ | Hlu-Mir-216_5p | ⇩ | ⇩ | |
Hlu-Mir-10-S22_5p | ↑ | ↑ | Hlu-Mir-277_3p | ↑ | |
Hlu-Mir-10-S23_5p | ↑ | ↑ | Hlu-Mir-279_3p | ⇩ | |
Hlu-Mir-10-S24_5p | ↑ | Hlu-Mir-317_3p | ⇩ | ||
Hlu-Mir-10-S25_5p | ↑ | Hlu-Mir-1985-S1_5p | ⇩ | ⇩ | |
Hlu-Mir-22-S1_3p | ⇩ | Hlu-Mir-1992_3p | ⇩ | ||
Hlu-Mir-29_3p | ⇩ | Hlu-Mir-2722-S1_3p | ↑ | ↑ | |
Hlu-Mir-33_5p | ⇩ |
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Vasiliev, G.V.; Ovchinnikov, V.Y.; Lisachev, P.D.; Bondar, N.P.; Grinkevich, L.N. The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum. Int. J. Mol. Sci. 2023, 24, 301. https://doi.org/10.3390/ijms24010301
Vasiliev GV, Ovchinnikov VY, Lisachev PD, Bondar NP, Grinkevich LN. The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum. International Journal of Molecular Sciences. 2023; 24(1):301. https://doi.org/10.3390/ijms24010301
Chicago/Turabian StyleVasiliev, Gennady V., Vladimir Y. Ovchinnikov, Pavel D. Lisachev, Natalia P. Bondar, and Larisa N. Grinkevich. 2023. "The Expression of miRNAs Involved in Long-Term Memory Formation in the CNS of the Mollusk Helix lucorum" International Journal of Molecular Sciences 24, no. 1: 301. https://doi.org/10.3390/ijms24010301