Monochromatic Green Light Stimulation during Incubation Alters Hepatic Glucose Metabolism That Improves Embryonic Development in Yangzhou Goose Eggs
Abstract
:1. Introduction
2. Results
2.1. Hatching Time and Hatching Performance
2.2. Embryonic Development
2.3. Plasma GH and IGF-1 Levels
2.4. Expression of Genes Associated with Leg Muscle Development
2.5. Global Transcriptomics Profiles in the Embryonic Livers
2.6. Global Metabolomic Profiles in Embryonic Livers
2.7. Integrated Analyses of the Metabolomics and Transcriptomics Data
2.8. Biochemical Index Related to Glucose Metabolism
3. Discussion
4. Material and Methods
4.1. Eggs and Experiments
4.2. Tissue Preparation
4.3. Histological Assessment
4.4. Transcriptomic Analysis
4.5. Metabolomics Analysis
4.6. Quantitative Real-Time PCR (qRT-PCR)
4.7. Biochemical Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Accession Number | Primer Sequence (5′–3′) | Fragment Size (bp) |
---|---|---|---|
β-Actin | XM_048058703.1 | TGACGCAGATCATGTTTGAGA GCAGAGCGTAGCCCTCATAG | 159 |
GAPDH | XM_013199522.2 | GCTGATGCTCCCATGTTCGTGAT GTGGTGCAAGAGGCATTGCTGAC | 86 |
TNNC1 | XM_013191946.2 | GGAGCTGCAGGAGATGATTGA CGAGGTCGATGTAGCCATCAG | 179 |
IGFBP4 | XM_048053219.1 | AGCATCCATGACCGCAAATG CTGTTGCGCATCTTGTTGCC | 74 |
ACTC1 | XM_048058696.1 | CCAGGGTGTTATGGTTGGCA | 218 |
TAGGGTTCAAGGGGGCTTCT | |||
SERPINB5 | XM_013187398.2 | ACTCACCCCCGAGACACTAT | 120 |
TCCAGAAGTGGCTTCAGGTC | |||
TTN | XM_048061293.1 | CAAAGCACCTCCACGAATGC CTGTCGTGCCATTTGAGCTT | 88 |
MYO7B | XM_013188025.2 | GAGGGACGCATTTGTGAAGG GGTGCACGAAGAACTGCTGA | 221 |
SERPINA1 | XM_013177486.2 | GTCCAGCTGAGTATGGGCAA GTCAACAAATTTGCCATGGGT | 186 |
INS | XM_013191620.2 | GGCTCTCTACCTGGTGTGTG TCCTCATGTTGGAACGGCAG | 129 |
MYH7B | XM_048080687.1 | AGCCCCGTCCGGATAAAAAG | 164 |
GGCCAGAAGCTTGTTTTGGG | |||
SCD5 | XM_013190576.2 | TGTGCTTTGTGATCCCCACC AGGAAGTAGGCGTTCCACAG | 70 |
FGF10 | XM_013181803.2 | TGTCTTCTGTGCCTGTCACC ATGCTGAAGGGGCAGTTCTC | 262 |
GNA14 | XM_013195956.2 | GGAGGGAGTACCAGCTCTCA TGGCACAAAGGAGGGCATAG | 80 |
MyoG | XM_048070946.1 | CGCCGCCTGAAGAAGGTGAA CCTGCTGGTTGAGGGTGCTGA | 154 |
MyoD | XM_013177726.2 | CATCCGCTACATCGAGAGCC ACTCCATCATGCCATCGGAG | 134 |
MYF5 | XM_048078887.1 | TGCAAAGCCTGCAAGAGAAAG GTCTCAAACGCCTGGTTCAC | 101 |
MRF4 | XM_048078889.1 | GGTTGTTCCTCGGGGTGTTTTC CTCCTTCTCCCCGTCCAAGTAG | 127 |
Pax3 | XM_048063046.1 | TTCACCTCAGGTAATGGGACTCTTG TGTAGGCAGGCTGTGTAAACTCTTCA | 169 |
Pax7 | XM_048048726.1 | CCTGGGCGACAAAGGTAA GCTCAGCGGTGAAAGTGG | 110 |
MSTN | XM_013178647.2 | GGCTCTTGATGACGGTAG CTTGTTCCAGACGCAGTT | 143 |
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Chen, Z.; Qu, X.; Feng, C.; Guo, B.; Zhu, H.; Yan, L. Monochromatic Green Light Stimulation during Incubation Alters Hepatic Glucose Metabolism That Improves Embryonic Development in Yangzhou Goose Eggs. Int. J. Mol. Sci. 2023, 24, 405. https://doi.org/10.3390/ijms24010405
Chen Z, Qu X, Feng C, Guo B, Zhu H, Yan L. Monochromatic Green Light Stimulation during Incubation Alters Hepatic Glucose Metabolism That Improves Embryonic Development in Yangzhou Goose Eggs. International Journal of Molecular Sciences. 2023; 24(1):405. https://doi.org/10.3390/ijms24010405
Chicago/Turabian StyleChen, Zhe, Xiaolu Qu, Chungang Feng, Binbin Guo, Huanxi Zhu, and Leyan Yan. 2023. "Monochromatic Green Light Stimulation during Incubation Alters Hepatic Glucose Metabolism That Improves Embryonic Development in Yangzhou Goose Eggs" International Journal of Molecular Sciences 24, no. 1: 405. https://doi.org/10.3390/ijms24010405