In Vivo Efficacy of Wound Healing under External (Bio)AgNCs Treatment: Localization Case Study in Liver and Blood Tissue
Abstract
:1. Introduction
2. Results
2.1. Animal Acclimatization, Body and Mouse Weights
2.2. Influence of LBPC-AgNCs Formulation on Wound Closure Rate
2.3. Uptake of Ag in Liver Tissue and Blood Samples
2.4. Viability of PBMC Cells–Ag Cytotoxicity Effect: Flow Cytometry Analysis
3. Discussion
4. Materials and Methods
4.1. AgNCs Description
4.2. Experimental Design: Animal Acclimatization, Body and Mice Weights, Wound Formation/Treatment and Biological Material Collection
4.3. Uptake of Ag in Liver Tissue and Blood Samples: Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Analysis
4.4. Viability of Peripheral Blood Mononuclear Cells—Ag Cytotoxicity Effect: Flow Cytometry Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Karimkhani, C.; Dellavalle, R.P.; Coffeng, L.E.; Flohr, C.; Hay, R.J.; Langan, S.M.; Nsoesie, E.O.; Ferrari, A.J.; Erskine, H.E.; Silverberg, J.I.; et al. Global Skin Disease Morbidity and Mortality. An Update from the Global Burden of Disease Study 2013. JAMA Dermatol. 2017, 153, 406–412. [Google Scholar] [CrossRef] [PubMed]
- Sen, C.K. Human Wounds and Its Burden: An Updated Compendium of Estimates. Adv. Wound Care 2019, 8, 39–48. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sen, C.K. Human Wound and Its Burden: Updated 2020 Compendium of Estimates. Adv. Wound Care 2021, 10, 281–292. [Google Scholar] [CrossRef] [PubMed]
- Sarsani, V.K.; Raghupathy, N.; Fiddes, I.T.; Armstrong, J.; Thibaud-Nissen, F.; Zinder, O.; Bolisetty, M.; Howe, K.; Hinerfeld, D.; Ruan, X.; et al. The Genome of C57BL/6J “Eve”, the Mother of the Laboratory Mouse Genome Reference Strain. G3 (Bethesda) 2019, 9, 1795–1805. [Google Scholar] [CrossRef] [Green Version]
- Chong, H.T.; Kopecki, Z.; Cowin, A.J. Lifting the Silver Flakes: The Pathogenesis and Management of Chronic Plaque Psoriasis. BioMed. Res. Int. 2013, 2013, 168321. [Google Scholar] [CrossRef]
- Keck, C.M.; Schwabe, K. Silver-Nanolipid Complex for Application to Atopic Dermatitis Skin: Rheological Characterization, in Vivo Efficiency and Theory of Action. J. Biomed. Nanotechnol. 2009, 5, 428–436. [Google Scholar] [CrossRef]
- van Saene, J.J.M.; Trooster, J.F.G.; Meulenhoff, A.M.C.; Lerk, C.F.; Bult, A. Release and Antimicrobial Activity of Silver Sulphadiazine from Different Creams. Burns 1987, 13, 123–130. [Google Scholar] [CrossRef] [Green Version]
- Gaiser, B.K.; Hirn, S.; Kermanizadeh, A.; Kanase, N.; Fytianos, K.; Wenk, A.; Haberl, N.; Brunelli, A.; Kreyling, W.G.; Stone, V. Effects of Silver Nanoparticles on the Liver and Hepatocytes in vitro. Toxicol. Sci. 2013, 131, 537–547. [Google Scholar] [CrossRef] [Green Version]
- Poznański, J.; Sołdacki, D.; Czarkowska-Pączek, B.; Bonna, A.; Kornasiewicz, O.; Krawczyk, M.; Bal, W.; Pączek, L. Cirrhotic Liver of Liver Transplant Recipients Accumulate Silver and Co-Accumulate Copper. Int. J. Mol. Sci. 2021, 22, 1782. [Google Scholar] [CrossRef]
- Wiemann, M.; Vennemann, A.; Blaske, F.; Sperling, M.; Karst, U. Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs. Nanomaterials 2017, 7, 441. [Google Scholar] [CrossRef]
- Valenzuela-Salas, L.M.; Girón-Vázquez, N.G.; García-Ramos, J.C.; Torres-Bugarín, O.; Gómez, C.; Pestryakov, A.; Villarreal-Gómez, L.J.; Toledano-Magaña, Y.; Bogdanchikova, N. Antiproliferative and Antitumour Effect of Nongenotoxic Silver Nanoparticles on Melanoma Models. Oxidative Med. Cell. Longev. 2020, 2019, 4528241. [Google Scholar] [CrossRef] [PubMed]
- Lu, W.; Senapati, D.; Wang, S.; Tovmachenko, O.; Singh, A.K.; Yu, H.; Ray, P.C. Effect of Surface Coating on the Toxicity of Silver Nanomaterials on Human Skin Keratinocytes. Chem. Phys. Lett. 2010, 487, 92–96. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Bastos, V.; Ferreira de Oliveira, J.M.P.; Brown, D.; Jonhston, H.; Malheiro, E.; Daniel-da-Silva, A.L.; Duarte, I.F.; Santos, C.; Oliveira, H. The Influence of Citrate or PEG Coating on Silver Nanoparticle Toxicity to a Human Keratinocyte Cell Line. Toxicol. Lett. 2016, 249, 29–41. [Google Scholar] [CrossRef] [PubMed]
- Railean-Plugaru, V.; Pomastowski, P.; Meller, K.; Złoch, M.; Rafińska, K.; Buszewski, B. Lactococcus Lactis as a Safe and Inexpensive Source of Bioactive Silver Composites. Appl. Microbiol. Biotechnol. 2017, 101, 7141–7153. [Google Scholar] [CrossRef] [Green Version]
- Railean-Plugaru, V.; Pomastowski, P.; Buszewski, B. Use of Lactobacillus Paracasei Isolated from Whey for Silver Nanocomposite Synthesis: Antiradical and Antimicrobial Properties against Selected Pathogens. J. Dairy Sci. 2021, 104, 2480–2498. [Google Scholar] [CrossRef]
- Kwon, H.B.; Lee, J.H.; Lee, S.H.; Lee, A.Y.; Choi, J.S.; Ahn, Y.S. A Case of Argyria Following Colloidal Silver Ingestion. Ann. Dermatol. 2009, 21, 308–310. [Google Scholar] [CrossRef] [Green Version]
- Król-Górniak, A.; Rafińska, K.; Monedeiro, F.; Pomastowski, P.; Buszewski, B. Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles. Int. J. Mol. Sci. 2021, 22, 9529. [Google Scholar] [CrossRef]
- Vuković, B.; Cvetić, Ž.; Bendelja, K.; Barbir, R.; Milić, M.; Dobrošević, B.; Šerić, V.; Vinković Vrček, I. In Vitro Study on the Immunomodulatory Effects of Differently Functionalized Silver Nanoparticles on Human Peripheral Blood Mononuclear Cells. J. Biol. Inorg. Chem. 2021, 26, 817–831. [Google Scholar] [CrossRef]
- Wei, L.; Lu, J.; Xu, H.; Patel, A.; Chen, Z.S.; Chen, G. Silver Nanoparticles: Synthesis, Properties, and Therapeutic Applications. Drug Discov. Today 2015, 20, 595–601. [Google Scholar] [CrossRef] [Green Version]
- Kim, W.Y.; Kim, J.; Park, J.D.; Ryu, H.Y.; Yu, I.J. Histological Study of Gender Differences in Accumulation of Silver Nanoparticles in Kidneys of Fischer 344 Rats. J. Toxicol. Environ. Health–Part A Curr. Issues 2009, 72, 1279–1284. [Google Scholar] [CrossRef]
- Recordati, C.; De Maglie, M.; Bianchessi, S.; Argentiere, S.; Cella, C.; Mattiello, S.; Cubadda, F.; Aureli, F.; D’Amato, M.; Raggi, A.; et al. Tissue Distribution and Acute Toxicity of Silver after Single Intravenous Administration in Mice: Nano-Specific and Size-Dependent Effects. Part. Fibre Toxicol. 2016, 13, 12. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Akahane, T.; Tsukada, S. Electron-Microscopic Observation on Silver Deposition in Burn Wounds Treated with Silver Sulphadiazine Cream. Burns 1982, 8, 271–273. [Google Scholar] [CrossRef] [PubMed]
- Tsipouras, N.; Rix, C.J.; Brady, P.H. Solubility of Silver Sulfadiazine in Physiological Media and Relevance to Treatment of Thermal Burns with Silver Sulfadiazine Cream. Clin. Chem. 1995, 41, 87–91. [Google Scholar] [CrossRef] [PubMed]
- Liao, C.; Li, Y.; Tjong, S.C. Bactericidal and Cytotoxic Properties of Silver Nanoparticles. Int. J. Mol. Sci. 2019, 20, 449. [Google Scholar] [CrossRef] [Green Version]
- Nešporová, K.; Pavlík, V.; Šafránková, B.; Vágnerová, H.; Odráška, P.; Žídek, O.; Císařová, N.; Skoroplyas, S.; Kubala, L.; Velebný, V. Effects of Wound Dressings Containing Silver on Skin and Immune Cells. Sci. Rep. 2020, 10, 15216. [Google Scholar] [CrossRef]
- Li, W.T.; Chang, H.W.; Yang, W.C.; Lo, C.; Wang, L.Y.; Pang, V.F.; Chen, M.H.; Jeng, C.R. Immunotoxicity of Silver Nanoparticles (AgNPs) on the Leukocytes of Common Bottlenose Dolphins (Tursiops Truncatus). Sci. Rep. 2018, 8, 5593. [Google Scholar] [CrossRef] [Green Version]
- Yuan, Y.G.; Peng, Q.L.; Ggurunathan, S. Silver Nanoparticles Enhance the Apoptotic Potential of Gemcitabine in Human Ovarian Cancer Cells: Combination Therapy for Effective Cancer Treatment. Int. J. Nanomed. 2017, 12, 6487–6502. [Google Scholar] [CrossRef] [Green Version]
- Orta-García, S.T.; Plascencia-Villa, G.; Ochoa-Martínez, A.C.; Ruiz-Vera, T.; Pérez-Vázquez, F.J.; Velázquez-Salazar, J.J.; Yacamán, M.J.; Navarro-Contreras, H.R.; Pérez-Maldonado, I.N. Analysis of Cytotoxic Effects of Silver Nanoclusters on Human Peripheral Blood Mononuclear Cells “in vitro”. J. Appl. Toxicol. 2015, 35, 1189–1199. [Google Scholar] [CrossRef]
- Antsiferova, A.A.; Kopaeva, M.Y.; Kochkin, V.N.; Kashkarov, P.K. Kinetics of Silver Accumulation in Tissues of Laboratory Mice after Long-Term Oral Administration of Silver Nanoparticles. Nanomaterials 2021, 11, 3204. [Google Scholar] [CrossRef]
- Railean-Plugaru, V.; Pomastowski, P.; Wypij, M.; Szultka-Młynska, M.; Rafińska, K.; Golińska, P.; Dahm, H.; Buszewski, B. Study of Silver Nanoparticles Synthesized by Acidophilic Strain of Actinobacteria Isolated from the of Picea Sitchensis Forest Soil. J. Appl. Microbiol. 2016, 120, 1250–1263. [Google Scholar] [CrossRef]
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Railean, V.; Buszewska-Forajta, M.; Rodzik, A.; Gołębiowski, A.; Pomastowski, P.; Buszewski, B. In Vivo Efficacy of Wound Healing under External (Bio)AgNCs Treatment: Localization Case Study in Liver and Blood Tissue. Int. J. Mol. Sci. 2023, 24, 434. https://doi.org/10.3390/ijms24010434
Railean V, Buszewska-Forajta M, Rodzik A, Gołębiowski A, Pomastowski P, Buszewski B. In Vivo Efficacy of Wound Healing under External (Bio)AgNCs Treatment: Localization Case Study in Liver and Blood Tissue. International Journal of Molecular Sciences. 2023; 24(1):434. https://doi.org/10.3390/ijms24010434
Chicago/Turabian StyleRailean, Viorica, Magdalena Buszewska-Forajta, Agnieszka Rodzik, Adrian Gołębiowski, Paweł Pomastowski, and Bogusław Buszewski. 2023. "In Vivo Efficacy of Wound Healing under External (Bio)AgNCs Treatment: Localization Case Study in Liver and Blood Tissue" International Journal of Molecular Sciences 24, no. 1: 434. https://doi.org/10.3390/ijms24010434