β-Hydroxy-β-Methylbutyric Acid Promotes Repair of Sheep Myoblast Injury by Inhibiting IL-17/NF-κB Signaling
Abstract
:1. Introduction
2. Results
2.1. Immunofluorescence of SMSCs
2.2. Cell Apoptosis and Proliferation
2.3. HMB Repairs Myoblast Injury by Blocking IL-17 and NF-κB Signaling
3. Discussion
4. Materials and Methods
4.1. Cell Isolation and Culture
4.2. Construction of Myoblast Injury Model
4.3. Detection of Cell Apoptosis by Flow Cytometry
4.4. EdU Assay
4.5. ELISA
4.6. Western Blot
4.7. RNA-Seq Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
Appendix B
Sample | Raw Reads | Raw Bases | Clean Reads | Clean Bases | Error Rate (%) | Q20(%) | Q30(%) | GC Content |
---|---|---|---|---|---|---|---|---|
(%) | ||||||||
NC1 | 6,100,7804 | 9,212,178,404 | 59,795,486 | 8,641,876,217 | 0.0252 | 97.86 | 94 | 53.63 |
NC2 | 5,404,5836 | 8,160,921,236 | 52,765,256 | 7,614,406,901 | 0.0256 | 97.69 | 93.62 | 53.8 |
NC3 | 5,465,8296 | 8,253,402,696 | 53,098,864 | 7,627,471,914 | 0.0254 | 97.77 | 93.87 | 53.77 |
H1 | 58,538,782 | 8,839,356,082 | 57,553,362 | 8,395,202,659 | 0.0251 | 97.93 | 94.16 | 53.55 |
H2 | 52,922,990 | 7,991,371,490 | 51,795,790 | 7,545,225,170 | 0.0257 | 97.66 | 93.54 | 53.5 |
H3 | 49,916,272 | 7,537,357,072 | 48,886,386 | 7,108,693,973 | 0.0249 | 97.98 | 94.32 | 53.38 |
D1 | 54,033,588 | 8,159,071,788 | 52,352,756 | 7,584,780,012 | 0.0253 | 97.8 | 93.95 | 53.79 |
D2 | 62,382,402 | 9,419,742,702 | 61,289,950 | 8,929,993,888 | 0.0252 | 97.88 | 94.03 | 53.47 |
D3 | 52,905,810 | 7,988,777,310 | 51,661,206 | 7,559,307,614 | 0.0253 | 97.8 | 93.91 | 53.46 |
HD1 | 54,294,954 | 8,198,538,054 | 53,026,950 | 7,734,884,116 | 0.0255 | 97.75 | 93.78 | 53.74 |
HD2 | 52,512,512 | 7,929,389,312 | 51,447,990 | 7,544,672,078 | 0.0251 | 97.93 | 94.18 | 53.33 |
HD3 | 51934726 | 7,842,143,626 | 50,781,802 | 7,445,053,103 | 0.025 | 97.93 | 94.21 | 53.39 |
D+H1 | 54,951,310 | 8,297,647,810 | 53,563,784 | 7,798,454,682 | 0.0255 | 97.72 | 93.71 | 53.69 |
D+H2 | 52,651,856 | 7,950,430,256 | 50,706,446 | 7,391,244,697 | 0.0256 | 97.67 | 93.66 | 53.6 |
D+H3 | 50,439,524 | 7,616,368,124 | 48,796,802 | 7,106,788,337 | 0.0254 | 97.8 | 93.9 | 53.71 |
DH1 | 57,570,642 | 8,693,166,942 | 56,450,908 | 8,226,601,480 | 0.025 | 97.94 | 94.22 | 53.38 |
DH2 | 57,144,710 | 8,628,851,210 | 55,878,600 | 8,101,309,031 | 0.0251 | 97.9 | 94.12 | 53.5 |
DH3 | 46,672,916 | 7,047,610,316 | 45,545,154 | 6,640,024,249 | 0.0252 | 97.85 | 94.06 | 53.59 |
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Medium | Ingredients |
---|---|
Complete medium | data High-glucose medium + 10%FBS + 1% penicillin/streptomycin (P/S) |
Myoblast Differentiation medium | data High-glucose medium + 2%HS |
Storage and transport | PBS + 5%P/S |
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Zheng, J.; Li, B.; Yan, Y.; Huang, X.; Zhang, E. β-Hydroxy-β-Methylbutyric Acid Promotes Repair of Sheep Myoblast Injury by Inhibiting IL-17/NF-κB Signaling. Int. J. Mol. Sci. 2023, 24, 444. https://doi.org/10.3390/ijms24010444
Zheng J, Li B, Yan Y, Huang X, Zhang E. β-Hydroxy-β-Methylbutyric Acid Promotes Repair of Sheep Myoblast Injury by Inhibiting IL-17/NF-κB Signaling. International Journal of Molecular Sciences. 2023; 24(1):444. https://doi.org/10.3390/ijms24010444
Chicago/Turabian StyleZheng, Juan, Bo Li, Yiting Yan, Xiaoyu Huang, and Enping Zhang. 2023. "β-Hydroxy-β-Methylbutyric Acid Promotes Repair of Sheep Myoblast Injury by Inhibiting IL-17/NF-κB Signaling" International Journal of Molecular Sciences 24, no. 1: 444. https://doi.org/10.3390/ijms24010444