Genome-Wide Characterization and Comparative Genomic Analysis of the Serpin Gene Family in Microsporidian Nosema bombycis
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification of Serpin Gene Family Members
2.2. Multiple Sequence Alignment and Conserved Motifs of NbSPN Proteins
2.3. Phylogenetic Analysis of NbSPNs
2.4. Genome Distribution of NbSPNs
2.5. Comparative Genome Analysis of the Serpin Genes in Microsporidia
2.6. Phylogenetic Analysis to Determine the Evolution Position of the N. bombycis Serpins
2.7. Expression Profiles of NbSPNs in Infected BmE-SWU1 Cells
2.8. Expression Profiles of NbSPN Genes in Infected Silkworm Tissues
3. Discussion
4. Materials and Methods
4.1. Silkworms and N. bombycis
4.2. Sequence Database Mining
4.3. Chromosomal Location and Gene Structure Analysis
4.4. Multiple Alignments and Phylogenetic Analysis
4.5. Oral Infection of Silkworms by N. bombycis Spores
4.6. Real-Time Quantitative PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID in SilkPathDB | GenBank Accession | Protein Length (Aa) | Signal Peptide (Aa) | MW 1 (kDa) | pI 2 | Inhibitory 3 |
---|---|---|---|---|---|---|---|
NbSPN1 | NBO_13g0021 | EOB14844 | 349 | none | 40.82 | 8.59 | + |
NbSPN2 | NBO_18g0004 | EOB14677 | 405 | 1–23 | 47.96 | 7.87 | + |
NbSPN3 | NBO_18g0021 | EOB14694 | 405 | 1–23 | 47.88 | 8.80 | + |
NbSPN4 | NBO_19g0007 | EOB14654 | 390 | 1–20 | 45.04 | 8.62 | + |
NbSPN5 | NBO_19g0009 | EOB14656 | 350 | none | 41.22 | 7.79 | + |
NbSPN6 | NBO_34g0030 | EOB14180 | 395 | 1–19 | 46.98 | 5.79 | + |
NbSPN7 | NBO_34g0036 | EOB14186 | 295 | none | 34.86 | 6.60 | + |
NbSPN8 | NBO_35g0002 | EOB14148 | 145 | none | 16.78 | 5.93 | + |
NbSPN9 | NBO_39gi001 | - | 392 | 1–27 | 46.05 | 6.71 | + |
NbSPN10 | NBO_42g0006 | EOB14016 | 395 | 1–19 | 46.88 | 6.56 | + |
NbSPN11 | NBO_44g0004 | EOB13986 | 168 | none | 19.61 | 5.57 | − |
NbSPN12 | NBO_44g0006 | EOB13988 | 109 | none | 12.98 | 5.07 | − |
NbSPN13 | NBO_124g0001 | EOB13192 | 390 | 1–25 | 45.12 | 6.69 | + |
NbSPN14 | NBO_372g0002 | - | 384 | 1–19 | 44.89 | 5.65 | + |
NbSPN15 | NBO_1038g0001 | EOB11591 | 132 | none | 15.23 | 4.47 | + |
NbSPN16 | NBO_1125g0001 | EOB11493 | 292 | none | 34.57 | 6.13 | + |
NbSPN17 | NBO_1147g0001 | EOB11479 | 206 | none | 24.17 | 5.56 | + |
NbSPN18 | NBO_1569g0001 | EOB11151 | 390 | none | 45.28 | 8.58 | + |
NbSPN19 | NBO_1570g0002 | EOB11150 | 181 | none | 21.10 | 9.32 | + |
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Ran, M.; Shi, Y.; Li, B.; Xiang, H.; Tao, M.; Meng, X.; Li, T.; Li, C.; Bao, J.; Pan, G.; et al. Genome-Wide Characterization and Comparative Genomic Analysis of the Serpin Gene Family in Microsporidian Nosema bombycis. Int. J. Mol. Sci. 2023, 24, 550. https://doi.org/10.3390/ijms24010550
Ran M, Shi Y, Li B, Xiang H, Tao M, Meng X, Li T, Li C, Bao J, Pan G, et al. Genome-Wide Characterization and Comparative Genomic Analysis of the Serpin Gene Family in Microsporidian Nosema bombycis. International Journal of Molecular Sciences. 2023; 24(1):550. https://doi.org/10.3390/ijms24010550
Chicago/Turabian StyleRan, Maoshuang, Yulian Shi, Boning Li, Heng Xiang, Meilin Tao, Xianzhi Meng, Tian Li, Chunfeng Li, Jialing Bao, Guoqing Pan, and et al. 2023. "Genome-Wide Characterization and Comparative Genomic Analysis of the Serpin Gene Family in Microsporidian Nosema bombycis" International Journal of Molecular Sciences 24, no. 1: 550. https://doi.org/10.3390/ijms24010550