Silver Nanoparticles Modified by Carbosilane Dendrons and PEG as Delivery Vectors of Small Interfering RNA
Abstract
:1. Introduction
2. Results
2.1. AgNP-siRNA Complexation
2.1.1. Changes in the Secondary Structure of RNA in the Presence of AgNP
2.1.2. Determination of the Formation of AgNP-siRNA Complexes
2.1.3. Estimation of the Surface Charge of AgNP-siRNA Complexes
2.1.4. Hydrodynamic Size of AgNP-siRNA Complexes
2.2. Toxicity of Silver Nanoparticles for Blood Cells
2.2.1. Hemotoxicity
2.2.2. PBMC Inhibition
2.3. Cellular Uptake of Nanoparticle Complex
2.4. Cytotoxicity Effects of AgNPs and Their Complexes with siMCL-1
2.5. Assessment of Cell Death Due to Exposure to AgNP-siRNA Complexes
3. Discussion
4. Materials and Methods
4.1. Small Interfering RNA
4.2. Silver Nanoparticles
4.3. Analysis of Complexes
4.3.1. Gel-Electrophoresis
4.3.2. Circular Dichroism
4.3.3. Zeta-Potential and Dynamic Light Scattering
4.4. Evaluation of Toxicity of Nanoparticles for Blood Cells
4.4.1. Hemolytic Activity of Silver Nanoparticles
4.4.2. PBMC Inhibition
4.5. Cell Line Cultures
4.6. Cellular Uptake
4.7. Cytotoxicity Studies
4.8. Apoptosis-Necrosis Test
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dendron Generation | Group Name | Dendron/PEG Molar Ratio | ||
---|---|---|---|---|
3:1 | 1:1 | 1:3 | ||
G1 | AgNP-G1 | 1aAg | 1bAg | 1cAg |
G2 | AgNP-G2 | 2aAg | 2bAg | 2cAg |
G3 | AgNP-G3 | 3aAg | 3bAg | 3cAg |
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Abashkin, V.; Pędziwiatr-Werbicka, E.; Horodecka, K.; Zhogla, V.; Ulashchik, E.; Shmanai, V.; Shcharbin, D.; Bryszewska, M. Silver Nanoparticles Modified by Carbosilane Dendrons and PEG as Delivery Vectors of Small Interfering RNA. Int. J. Mol. Sci. 2023, 24, 840. https://doi.org/10.3390/ijms24010840
Abashkin V, Pędziwiatr-Werbicka E, Horodecka K, Zhogla V, Ulashchik E, Shmanai V, Shcharbin D, Bryszewska M. Silver Nanoparticles Modified by Carbosilane Dendrons and PEG as Delivery Vectors of Small Interfering RNA. International Journal of Molecular Sciences. 2023; 24(1):840. https://doi.org/10.3390/ijms24010840
Chicago/Turabian StyleAbashkin, Viktar, Elżbieta Pędziwiatr-Werbicka, Katarzyna Horodecka, Victoriya Zhogla, Egor Ulashchik, Vadim Shmanai, Dzmitry Shcharbin, and Maria Bryszewska. 2023. "Silver Nanoparticles Modified by Carbosilane Dendrons and PEG as Delivery Vectors of Small Interfering RNA" International Journal of Molecular Sciences 24, no. 1: 840. https://doi.org/10.3390/ijms24010840