A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Ten Small Molecule Compounds Are Screened as Potential ABA Analogues through Virtual Screening Techniques
2.2. SLG1 Inhibits the Seedlings Growth of A. thaliana and Activates the Expression of ABA-Related Genes
2.3. SLG1 Reduces Water Loss and Enhances Drought Resistance
2.4. SLG1 Is a Potential agonist of Multiple ABA Receptors
2.5. Structural Basis of SLG1 Recognition by ABA Receptors
2.6. Molecular Dynamics Simulations of SLG1 Recognition by ABA Receptors
3. Discussion
4. Materials and Methods
4.1. Preparation Crystal Structure of PYL1 Protein
4.2. Preparation of Small Molecule Compounds
4.3. Molecular Docking Screening
4.4. Pharmacophore Screening
4.5. Phenotypic Assays
4.6. Gene Expression Analysis
4.7. Stability Measurement of ABA and SLG1
4.8. Yeast Two-Hybrid Assay
4.9. Agonist/Receptor-Mediated Phosphatase Inhibition Assays
4.10. Molecular Docking
4.11. Molecular Dynamics Simulations
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, R.; Liang, G.; Gong, J.; Wang, J.; Zhang, Y.; Hao, Z.; Li, G. A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2023, 24, 8783. https://doi.org/10.3390/ijms24108783
Liu R, Liang G, Gong J, Wang J, Zhang Y, Hao Z, Li G. A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences. 2023; 24(10):8783. https://doi.org/10.3390/ijms24108783
Chicago/Turabian StyleLiu, Ruiqi, Guoyan Liang, Jiaxin Gong, Jiali Wang, Yanjie Zhang, Zhiqiang Hao, and Guanglin Li. 2023. "A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana" International Journal of Molecular Sciences 24, no. 10: 8783. https://doi.org/10.3390/ijms24108783