Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics
Abstract
:1. Introduction
2. Results
2.1. Effect of the Medium on CD11b Expression
2.2. Effect of fMLP on CD11b Expression
2.3. Effect of VEGF on CD11b Expression
3. Discussion
4. Materials and Methods
4.1. Study Groups
4.2. Bronchodilation Test
4.3. Flow Cytometry Method
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Incubation Placement | Asthmatics | Controls | ||
---|---|---|---|---|
All Examined Patients | Group with Reversible Airway Narrowing | Group with Irreversible Airway Narrowing | ||
Patient background (Pb—medium) | 332.21 (135.67; 725.60) | 300.27 (151.24; 676.32) | 347.56 (135.67; 725.60) | 297.76 (8.42; 886.62) |
Positive control (Pc—fMLP) | 393.10 (206.18; 1619.68) | 394.15 (206.18; 905.30) | 392.05 (218.74; 1619.68) | 405.98 (17.74; 2181.30) |
VEGF (250 ng/mL) | 350.86 * (127.59; 1003.32) | 335.10 (127.59; 668.25) | 322.68 (144.21; 1003.32) | 309.48 * (5.66; 1158.14) |
VEGF (500 ng/mL) | 331.53 (157.80; 1077.13) | 323.02 (163.03; 764.08) | 329.51 (157.80; 1077.13) | 327.22 (9.13; 1507.55) |
Variables | Asthmatics | Controls | |
---|---|---|---|
Reversible Airway Narrowing | Irreversible Airway Narrowing | ||
Participants (N) | 39 | 39 | 40 |
Female gender (%N) | 28 (71.79%) | 24 (61.54%) | 26 (65%) |
Age (years) mean ± SD | 50.0 ± 12.7 | 51.7 ± 10.3 | 48.0 ± 13.7 |
Age (years) Me (min.; max.) | 51.0 (23; 69) | 54.0 (24; 69) | 52.5 (20; 70) |
Asthma duration (years) mean ± SD | 10.04 ± 8.28 | 21.94 ± 11.88 | - |
inhGKS (%N) | 36 (92.31%) | 25 (64.10%) | - |
LABA (%N) | 37 (94.92%) | 26 (66.67%) | - |
Smoker—current or former (%N) | 5 (12.82%) | 14 (35.9%) | 11 (27.50%) |
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Gomułka, K.; Tota, M.; Brzdąk, K. Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics. Int. J. Mol. Sci. 2023, 24, 8880. https://doi.org/10.3390/ijms24108880
Gomułka K, Tota M, Brzdąk K. Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics. International Journal of Molecular Sciences. 2023; 24(10):8880. https://doi.org/10.3390/ijms24108880
Chicago/Turabian StyleGomułka, Krzysztof, Maciej Tota, and Kacper Brzdąk. 2023. "Effect of VEGF Stimulation on CD11b Receptor on Peripheral Eosinophils in Asthmatics" International Journal of Molecular Sciences 24, no. 10: 8880. https://doi.org/10.3390/ijms24108880