Insights into the Alcyoneusvirus Adsorption Complex
Abstract
:1. Introduction
2. Results
2.1. In Silico Analysis of RaK2 TFPs
2.2. Production of Recombinant RaK2 TFPs
2.3. Depolymerase Activity Test
2.4. Western Blot Analysis of Antisera Raised against Recombinant RaK2 TFPs
2.5. RaK2 Inhibition with Antisera
2.6. Adsorption Inhibition Assay
2.7. AFM and Immunogold-TEM Analyses
3. Discussion
4. Materials and Methods
4.1. Bioinformatics Analysis
4.2. Phage, Bacterial Strains, and Growth Conditions
4.3. Plasmid Construction
4.4. Protein Production and Purification
4.5. Depolymerase Activity Assay
4.6. RaK2 Adsorption Inhibition with Recombinant Proteins
4.7. Antisera Production
4.8. RaK2 Adsorption Inhibition with Policlonal Antiserum
4.9. Immunogold Labeling and TEM Analysis
4.10. AFM Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Recombinant Protein | MW, kDa 1 | MW, kDa 2 | IPTG, mM | Temperature, °C | Time, h | E. coli Strain |
---|---|---|---|---|---|---|
098C | 62.9 | 26.3 * | 1.0 | 22 | 18 | Rosetta DE3 |
526 | 63.1 | 66.1 | 1.0 | 20 | 18 | Rosetta DE3 |
527C | 79.5 | 21.6 * | 0.5 | 30 | 18 | Rosetta DE3 |
528N | 121.8 | 46.3 * | 0.8 | 25 | 18 | HMS174 DE3 |
529 | 62.5 | 64.9 | 0.5 | 17 | 17 | Rosetta DE3 |
530C | 86.1 | 29.7 * | 0.5 | 30 | 18 | Rosetta DE3 |
531 | 97.8 | 100.3 | 0.5 | 17 | 19 | Rosetta DE3 |
532 | 87.3 | 89.8 | 1.0 | 18 | 17 | HMS174 DE3 |
533 | 82.8 | 85.4 | 0.5 | 30 | 21 | Rosetta DE3 |
534C | 70.8 | 28.4 * | 0.5 | 30 | 18 | Rosetta DE3 |
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Noreika, A.; Rutkiene, R.; Dumalakienė, I.; Vilienė, R.; Laurynėnas, A.; Povilonienė, S.; Skapas, M.; Meškys, R.; Kaliniene, L. Insights into the Alcyoneusvirus Adsorption Complex. Int. J. Mol. Sci. 2023, 24, 9320. https://doi.org/10.3390/ijms24119320
Noreika A, Rutkiene R, Dumalakienė I, Vilienė R, Laurynėnas A, Povilonienė S, Skapas M, Meškys R, Kaliniene L. Insights into the Alcyoneusvirus Adsorption Complex. International Journal of Molecular Sciences. 2023; 24(11):9320. https://doi.org/10.3390/ijms24119320
Chicago/Turabian StyleNoreika, Algirdas, Rasa Rutkiene, Irena Dumalakienė, Rita Vilienė, Audrius Laurynėnas, Simona Povilonienė, Martynas Skapas, Rolandas Meškys, and Laura Kaliniene. 2023. "Insights into the Alcyoneusvirus Adsorption Complex" International Journal of Molecular Sciences 24, no. 11: 9320. https://doi.org/10.3390/ijms24119320