Comparative Analysis of Water-Soluble Polysaccharides from Dendrobium Second Love ‘Tokimeki’ and Dendrobium nobile in Structure, Antioxidant, and Anti-Tumor Activity In Vitro
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Purification of Polysaccharides
2.2. Molecular Weight (Mw) Determination and Infrared Spectroscopy
2.3. Monosaccharide and Methylation Analysis
2.4. NMR Analysis
2.5. Antioxidant Activity
2.6. In Vitro Anti-Proliferative Activity
3. Discussion
4. Materials and Methods
4.1. Materials and Chemicals
4.2. Composition and Structure Analysis of Polysaccharide
4.2.1. Extraction and Purification of Polysaccharide
4.2.2. Determination of Purity, Uronic Acid, and Molecular Weight
4.2.3. Determination of Monosaccharide Composition
4.2.4. Methylation Analysis
4.2.5. IR and NMR Spectroscopy
4.3. Bioactivities of Polysaccharide
4.3.1. Antioxidant Activity Test
4.3.2. Proliferative Inhibition of SPC-A-1 Cells In Vitro
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Retention Time (min) | Mass Fraction (m/z) | Linkage Pattern | Molar Ratio | |
---|---|---|---|---|
DHPP-Is | DNPP-Is | |||
11.967 | 59, 71, 87, 102, 113, 118, 129, 145, 161, 162, 205 | T-Manp | 2.40 | 1.82 |
14.271 | 59, 71, 87, 99, 102, 113, 118, 129, 131, 162, 233 | 1,4-Linked Manp | 63.32 | 82.67 |
14.421 | 59, 71, 87, 99, 102, 113, 118, 129, 131, 162, 233 | 1,4-Linked Glcp | 35.99 | 15.34 |
16.200 | 59, 71, 85, 99, 102, 118, 127, 142, 162, 201, 261 | 1,4,6-Linked Manp | 0.28 | 0.15 |
Sugar Residues | Ratio | 1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|---|---|
→4)-β-D-Glcp-(1→ | 2.4 | δH | 4.53 | 3.36 | 3.61 | 3.71 | 3.65 | - |
δC | 103.48 | 73.56 | 75.58 | 79.47 | 75.93 | - | ||
→4)-α-D-Glcp-(1→ | 5.2 | δH | 5.42 | 3.65 | 3.98 | 3.86 | 3.68 | 3.78 |
δC | 100.28 | 72.19 | 73.98 | 71.83 | 77.36 | 61.14 | ||
→4)-β-D-Manp-(1→ | 8.2 | δH | 4.76 | 4.08 | 3.96 | 3.65 | 3.99 | 3.87, 3.84 |
δC | 100.80 | 70.78 | 71.74 | 74.02 | 70.38 | 61.08 | ||
→4)-2-O-acetyl-β-D-Manp-(1→ | 1.5 | δH | 4.98 | 5.51 | 4.02 | 3.98 | 3.67 | - |
δC | 99.28 | 72.19 | 74.00 | 71.84 | 77.33 | - | ||
→4)-3-O-acetyl-β-D-Manp-(1→ | 1.0 | δH | 5.41 | 4.21 | 5.12 | 4.05 | 3.62 | - |
δC | 100.23 | 69.27 | 73.94 | 70.72 | 75.52 | - |
Sugar Residues | Ratio | 1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|---|---|
→4)-β-D-Glcp-(1→ | 1.2 | δH | 4.53 | 3.36 | 3.71 | 4.27 | 3.65 | 3.83, 4.00 |
δC | 103.13 | 70.63 | 72.37 | 74.53 | 72.37 | 61.09 | ||
→4)-α-D-Glcp-(1→ | 0.8 | δH | 5.42 | 3.65 | 3.98 | 3.86 | 3.68 | 3.78 |
δC | 100.38 | 72.19 | 73.98 | 71.83 | 77.36 | 61.14 | ||
→4)-β-D-Manp-(1→ | 3.6 | δH | 4.76 | 3.57 | 3.83 | 4.14 | 3.83 | 3.92, 3.77 |
δC | 100.75 | 75.64 | 72.05 | 70.59 | 77.13 | 61.09 | ||
→4)-2-O-acetyl-β-D-Manp-(1→ | 1.6 | δH | 4.96 | 5.52 | 4.03 | 3.82 | 3.83 | - |
δC | 99.86 | 72.24 | 73.84 | 72.34 | 77.09 | 61.09 | ||
→4)-3-O-acetyl-β-D-Manp-(1→ | 0.8 | δH | 5.41 | 4.21 | 5.12 | 4.04 | 3.82 | - |
δC | 100.41 | 69.27 | 73.94 | 70.72 | 75.52 | 61.09 | ||
→4)-2,3-O-acetyl-β-D-Manp-(1→ | 0.2 | δH | 5.42 | 5.53 | 5.24 | 4.10 | 3.77 | 3.94 |
δC | 100.61 | 70.44 | 72.31 | 69.26 | 75.61 | 61.09 |
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Ye, G.; Zhang, J.; Xu, X.; Zeng, C.; Ye, Q.; Wang, Z. Comparative Analysis of Water-Soluble Polysaccharides from Dendrobium Second Love ‘Tokimeki’ and Dendrobium nobile in Structure, Antioxidant, and Anti-Tumor Activity In Vitro. Int. J. Mol. Sci. 2023, 24, 10361. https://doi.org/10.3390/ijms241210361
Ye G, Zhang J, Xu X, Zeng C, Ye Q, Wang Z. Comparative Analysis of Water-Soluble Polysaccharides from Dendrobium Second Love ‘Tokimeki’ and Dendrobium nobile in Structure, Antioxidant, and Anti-Tumor Activity In Vitro. International Journal of Molecular Sciences. 2023; 24(12):10361. https://doi.org/10.3390/ijms241210361
Chicago/Turabian StyleYe, Guangying, Jinhui Zhang, Xiaoli Xu, Canbiao Zeng, Qingsheng Ye, and Zaihua Wang. 2023. "Comparative Analysis of Water-Soluble Polysaccharides from Dendrobium Second Love ‘Tokimeki’ and Dendrobium nobile in Structure, Antioxidant, and Anti-Tumor Activity In Vitro" International Journal of Molecular Sciences 24, no. 12: 10361. https://doi.org/10.3390/ijms241210361