A Surprising Diversity of Xyloglucan Endotransglucosylase/Hydrolase in Wheat: New in Sight to the Roles in Drought Tolerance
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification of the TaXTH Family and Phylogenetic Relationships
2.2. Chromosomal Distribution and Duplication Process of TaXTHs
2.3. Gene Structure of TaXTHs and Structure-Based Protein Conservative Sequence Analysis
2.4. Expression Pattern of the XTH Genes in the Wheat Germinated Seeds
2.5. Expression Pattern of TaXTHs in Wheat Roots and Shoots under Drought Stress
2.6. Stable Transgenic Arabidopsis with Overexpression of TaXTH12.5a
2.7. Transgenic Arabidopsis Exhibits Drought Tolerance during the Seedling Stage
2.8. Transgenic Arabidopsis Exhibits Drought Tolerance during the Vegetative Stage
3. Discussion
3.1. Characterization of the TaXTH Gene Family
3.2. The Expression Patterns of TaXTHs Were Regulated by Drought
3.3. The Biological Function of TaXTH12.5a in Arabidopsis under Drought Stress
4. Materials and Methods
4.1. Identification, Chromosomal Location, and Structural Organization of TaXTHs in Wheat
4.2. TaXTH Protein Sequence Alignment and Phylogenetic Analysis
4.3. Plant Growth Conditions and Treatments
4.4. Structure-Based Sequence Alignment, Construction of Conserved Motifs, and Promoter Analysis
4.5. RNA Isolation, cDNA Transcription, and Quantitative Real-Time PCR for the TaXTH Genes
4.6. Construction of the pCAMBIA1301-TaXTH12.5a Vector, Agrobacterium-Mediated Arabidopsis Transformation and Segregation of Posterity
4.7. DNA Extraction and PCR Identification of Transgenic Plants
4.8. QuantStudio 3D Digital PCR Analysis for the Arabidopsis TaXTH12.5a Transgenic Copy Number
4.9. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Han, J.; Liu, Y.; Shen, Y.; Li, W. A Surprising Diversity of Xyloglucan Endotransglucosylase/Hydrolase in Wheat: New in Sight to the Roles in Drought Tolerance. Int. J. Mol. Sci. 2023, 24, 9886. https://doi.org/10.3390/ijms24129886
Han J, Liu Y, Shen Y, Li W. A Surprising Diversity of Xyloglucan Endotransglucosylase/Hydrolase in Wheat: New in Sight to the Roles in Drought Tolerance. International Journal of Molecular Sciences. 2023; 24(12):9886. https://doi.org/10.3390/ijms24129886
Chicago/Turabian StyleHan, Junjie, Yichen Liu, Yiting Shen, and Weihua Li. 2023. "A Surprising Diversity of Xyloglucan Endotransglucosylase/Hydrolase in Wheat: New in Sight to the Roles in Drought Tolerance" International Journal of Molecular Sciences 24, no. 12: 9886. https://doi.org/10.3390/ijms24129886
APA StyleHan, J., Liu, Y., Shen, Y., & Li, W. (2023). A Surprising Diversity of Xyloglucan Endotransglucosylase/Hydrolase in Wheat: New in Sight to the Roles in Drought Tolerance. International Journal of Molecular Sciences, 24(12), 9886. https://doi.org/10.3390/ijms24129886