2-DG Regulates Immune Imbalance on the Titanium Surface after Debridement
Abstract
:1. Introduction
2. Results
2.1. Construction and Characterization of the Titanium Surface after Debridement
2.2. Titanium Surface after Debridement Results in Immune Imbalance
2.3. 2-DG Could Regulate the Pro-Inflammatory Immune Imbalance of Macrophages Induced by Titanium Surface after Debridement
2.4. 2-DG Could Regulate the Pro-Inflammatory Immune Imbalance of T Cells Induced by Titanium Surface after Debridement
3. Discussion
4. Materials and Methods
4.1. Titanium Discs
4.2. Bacterial Culture and Biofilm Formation
4.3. Debridement on Bacterial-Colonized Titanium Discs
4.4. Scanning Electron Microscopy (SEM) and Chemical Analyses
4.5. Cell Preparation and Culture
4.6. Flow Cytometry
4.7. RNA Extraction and qRT-PCR
4.8. Statistic Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, X.; Deng, S.; Xie, J.; Xu, C.; Huang, Z.; Huang, B.; Chen, Z.; Chen, S. 2-DG Regulates Immune Imbalance on the Titanium Surface after Debridement. Int. J. Mol. Sci. 2023, 24, 11431. https://doi.org/10.3390/ijms241411431
Liu X, Deng S, Xie J, Xu C, Huang Z, Huang B, Chen Z, Chen S. 2-DG Regulates Immune Imbalance on the Titanium Surface after Debridement. International Journal of Molecular Sciences. 2023; 24(14):11431. https://doi.org/10.3390/ijms241411431
Chicago/Turabian StyleLiu, Xingchen, Shudan Deng, Jiaxin Xie, Chunxin Xu, Zhuwei Huang, Baoxin Huang, Zhuofan Chen, and Shoucheng Chen. 2023. "2-DG Regulates Immune Imbalance on the Titanium Surface after Debridement" International Journal of Molecular Sciences 24, no. 14: 11431. https://doi.org/10.3390/ijms241411431