Involvement of Serotonergic Projections from the Dorsal Raphe to the Medial Preoptic Area in the Regulation of the Pup-Directed Paternal Response of Male Mandarin Voles
Abstract
:1. Introduction
2. Results
2.1. 5-HT Release in the MPOA upon Licking or Attacking
2.2. Verification of 5-HT Neuron Projections from the Ventral Part of the DR Region to the MPOA
2.3. Activities of 5-HTergic MPOA Projecting Neurons in the DR Increase upon the Licking of Pups
2.4. Chemogenetic Inhibition of the 5-HTergic Neural Projections Reduces the Licking Duration and the Latency to Attack Pups
2.5. Optogenetic Activation of 5-HTergic Neural Projections from DR to MPOA Increases the Licking Duration and Elongates the Latency to Attack Pups
3. Discussion
4. Material and Methods
4.1. Animals
4.2. Stereotaxic Surgery
4.3. Fiber Photometry
4.4. Immunofluorescence
4.5. Chemogenetics
4.6. Optogenetics
4.7. In Vitro Electrophysiology Recordings
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lv, Z.; Li, L.; Li, Y.; Zhang, L.; Guo, X.; Huang, C.; Hou, W.; Qu, Y.; Liu, L.; Li, Y.; et al. Involvement of Serotonergic Projections from the Dorsal Raphe to the Medial Preoptic Area in the Regulation of the Pup-Directed Paternal Response of Male Mandarin Voles. Int. J. Mol. Sci. 2023, 24, 11605. https://doi.org/10.3390/ijms241411605
Lv Z, Li L, Li Y, Zhang L, Guo X, Huang C, Hou W, Qu Y, Liu L, Li Y, et al. Involvement of Serotonergic Projections from the Dorsal Raphe to the Medial Preoptic Area in the Regulation of the Pup-Directed Paternal Response of Male Mandarin Voles. International Journal of Molecular Sciences. 2023; 24(14):11605. https://doi.org/10.3390/ijms241411605
Chicago/Turabian StyleLv, Zijian, Lu Li, Yin Li, Lizi Zhang, Xing Guo, Caihong Huang, Wenjuan Hou, Yishan Qu, Limin Liu, Yitong Li, and et al. 2023. "Involvement of Serotonergic Projections from the Dorsal Raphe to the Medial Preoptic Area in the Regulation of the Pup-Directed Paternal Response of Male Mandarin Voles" International Journal of Molecular Sciences 24, no. 14: 11605. https://doi.org/10.3390/ijms241411605