The Evaluation of Rac1 Signaling as a Potential Therapeutic Target of Alzheimer’s Disease
Abstract
:1. Introduction
2. Results
2.1. RacGAP Bcr Knockdown in Striatal/Accumbal Neurons Enhances Rac1-PAK Signaling
2.2. Bcr Knockdown in the NAc Enhances Aversive Learning
3. Discussion
4. Materials and Methods
4.1. Antibodies
4.2. Cell Culture and Transfection
4.3. Animal
4.4. Plasmid Construction
4.5. Primary Neuron Culture
4.6. Cell Ruffling Assay
4.7. Ex Vivo Sample Preparation
4.8. In Vivo Sample Preparation
4.9. SDS-PAGE Immunoblotting Assay
4.10. AAV Preparation
4.11. AAV Injection
4.12. Passive Avoidance Test
4.13. Immunohistochemical Analysis
4.14. Quantification and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, H.; Yamahashi, Y.; Riedl, M.; Amano, M.; Kaibuchi, K. The Evaluation of Rac1 Signaling as a Potential Therapeutic Target of Alzheimer’s Disease. Int. J. Mol. Sci. 2023, 24, 11880. https://doi.org/10.3390/ijms241511880
Wang H, Yamahashi Y, Riedl M, Amano M, Kaibuchi K. The Evaluation of Rac1 Signaling as a Potential Therapeutic Target of Alzheimer’s Disease. International Journal of Molecular Sciences. 2023; 24(15):11880. https://doi.org/10.3390/ijms241511880
Chicago/Turabian StyleWang, Huanhuan, Yukie Yamahashi, Marcel Riedl, Mutsuki Amano, and Kozo Kaibuchi. 2023. "The Evaluation of Rac1 Signaling as a Potential Therapeutic Target of Alzheimer’s Disease" International Journal of Molecular Sciences 24, no. 15: 11880. https://doi.org/10.3390/ijms241511880