Adlercreutzia equolifaciens Is an Anti-Inflammatory Commensal Bacterium with Decreased Abundance in Gut Microbiota of Patients with Metabolic Liver Disease
Abstract
:1. Introduction
2. Results
2.1. Decreased Abundance of A. equolifaciens in Microbiome of Liver Disease Patients NASH1 and NASH2
2.2. A. equolifaciens Encompasses Two Distinct Genomospecies with Strong Associations with Geography
2.3. A. equolifaciens Displays Anti-Inflammatory Properties In Vitro
2.4. A. equolifaciens Displays Anti-Inflammatory Effect In Vivo and Impacts Mice Metabolism
3. Discussion
3.1. Decreased Abundance of A. equolifaciens in the Microbiome of NAFLD/NASH Patients
3.2. A. equolifaciens Presence in Human Gut
3.3. A. equolifaciens Presents Anti-Inflammatory Properties
4. Materials and Methods
4.1. Metagenome-Wide Association Study
4.1.1. Study Population NASH1 Cohort
4.1.2. Study Population NASH2 Cohort
4.1.3. Reference Healthy Volunteer Populations Spanish MetaHIT
4.1.4. Analysis of MGS in NASH1, NASH2, and Healthy Cohort (MetaHit)
4.1.5. Data Availability
4.1.6. Sequencing Data Preprocessing
4.1.7. Gene Coverage Table Generation
4.1.8. Species-Level Taxonomic Profiling
4.1.9. Statistical Analysis
4.2. Population Genomics
4.2.1. Data Availability
4.2.2. Bioinformatics and Biostatistics
4.3. In Vitro Experiments
4.3.1. Cell Culture and Reagents
4.3.2. Commensal Strains and Preparation of Conditioned Media
4.3.3. Analyses of NF-κB Activation
4.3.4. Statistical Analysis
4.4. Animal Experiments
4.4.1. Clinical Cohort for Stool Selection
4.4.2. Preparation and Preservation of Fecal Transplants
4.4.3. Animal Experimentation
4.4.4. Preparation of A. equolifaciens for Gavage
4.4.5. 16S rRNA Sequencing Analysis
4.4.6. Short-Chain Fatty Acids Quantification of Cecal Contents
4.4.7. Plasma Assays
4.4.8. Real-Time Quantitative Polymerase Chain Reaction (qPCR)
4.4.9. Oral Glucose Tolerance Test (OGTT)
4.4.10. Statistical Analysis
5. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oñate, F.P.; Chamignon, C.; Burz, S.D.; Lapaque, N.; Monnoye, M.; Philippe, C.; Bredel, M.; Chêne, L.; Farin, W.; Paillarse, J.-M.; et al. Adlercreutzia equolifaciens Is an Anti-Inflammatory Commensal Bacterium with Decreased Abundance in Gut Microbiota of Patients with Metabolic Liver Disease. Int. J. Mol. Sci. 2023, 24, 12232. https://doi.org/10.3390/ijms241512232
Oñate FP, Chamignon C, Burz SD, Lapaque N, Monnoye M, Philippe C, Bredel M, Chêne L, Farin W, Paillarse J-M, et al. Adlercreutzia equolifaciens Is an Anti-Inflammatory Commensal Bacterium with Decreased Abundance in Gut Microbiota of Patients with Metabolic Liver Disease. International Journal of Molecular Sciences. 2023; 24(15):12232. https://doi.org/10.3390/ijms241512232
Chicago/Turabian StyleOñate, Florian Plaza, Célia Chamignon, Sebastian D. Burz, Nicolas Lapaque, Magali Monnoye, Catherine Philippe, Maxime Bredel, Laurent Chêne, William Farin, Jean-Michel Paillarse, and et al. 2023. "Adlercreutzia equolifaciens Is an Anti-Inflammatory Commensal Bacterium with Decreased Abundance in Gut Microbiota of Patients with Metabolic Liver Disease" International Journal of Molecular Sciences 24, no. 15: 12232. https://doi.org/10.3390/ijms241512232