Spectral Relaxation Imaging Microscopy II: Complex Dynamics
Abstract
:1. Introduction
2. Results and Materials and Methods
2.1. Theory of the Red-Fluorescence-Edge Phasor Ellipse Approach
2.1.1. Derivation of Initial and Relaxed States for Single-Spectral-Relaxation/Single-Lifetime Model
2.2. Complex Models of Spectral Relaxation (Two Spectral Relaxation Times and One Lifetime)
2.3. Interpretation of Motions by Projection onto Sub-Nanosecond–Super-Nanosecond Spectral Relaxation Correlation Times (IMPOSSE)
3. Discussion
4. Conclusions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Model | Input τs | Input GPi | Input GPf | Output Parameters |
---|---|---|---|---|
1 | 0.1 | 0.6 | −0.6 | 0.1, 0.6, −0.6 |
2 | 1 | 0.0 | −0.6 | 1, 0.0, −0.6 |
3 | 3 | −0.2 | −0.6 | 3, −0.2, −0.6 |
4 | 10 | 0.3 | −0.3 | 10, 0.3, −0.3 |
Input GPi; GPf | Input τ1; τ2 | Input A1; A2 | Ouput GPi; GPf | Output τs |
---|---|---|---|---|
0.6; −0.6 | 0.1; 3 | 0.2; 0.8 | 0.385; −0.598 | 2.91 |
0.6; −0.6 | 0.1; 3 | 0.4; 0.6 | 0.172; −0.596 | 2.78 |
0.6; −0.6 | 0.1; 3 | 0.6; 0.4 | −0.05; −0.594 | 2.56 |
0.6; −0.6 | 0.1; 3 | 0.8; 0.2 | −0.25; −0.594 | 2.06 |
0.6; −0.6 | 1; 3 | 0.2; 0.8 | 0.536; −0.594 | 2.67 |
0.6; −0.6 | 1; 3 | 0.4; 0.6 | 0.484; −0.590 | 2.31 |
0.6; −0.6 | 1; 3 | 0.6; 0.4 | 0.453; −0.590 | 1.91 |
0.6; −0.6 | 1; 3 | 0.8; 0.2 | 0.467; −0.593 | 1.48 |
0.6; −0.6 | 100; 3 | 0.2; 0.8 | 0.582; −0.397 | 3.22 |
0.6; −0.6 | 100; 3 | 0.4; 0.6 | 0.568; −0.195 | 3.61 |
0.6; −0.6 | 100; 3 | 0.6; 0.4 | 0.561; 0.003 | 4.35 |
0.6; −0.6 | 100; 3 | 0.8; 0.2 | 0.566; 0.185 | 6.53 |
Guess τs1 | τs2 | A1/A2 | Calculated GP | True GP |
---|---|---|---|---|
0.50 | 3.49 | 0.55/0.45 | −0.378 | −0.329 |
0.80 | 6.25 | 0.72/0.28 | −0.352 | −0.329 |
0.89 | 8.62 | 0.748/0.252 | −0.332 | −0.329 |
0.90 | 9.00 | 0.750/0.250 | −0.329 | −0.329 |
0.91 | 9.41 | 0.752/0.248 | −0.325 | −0.329 |
1.0 | 15.27 | 0.75/0.25 | −0.282 | −0.329 |
1.2 | 47.83 | 0.635/0.365 | −0.106 | −0.329 |
Phasor | Phasor | Model | GPi | GPf | τs1 | τs2 | A1 | τ | |
---|---|---|---|---|---|---|---|---|---|
interior | (0.276, 0.428) | (0.087, 0.4) | single | 0.76 | 0.35 | 2.55 | 0 | 0 | 3.63 |
interior | (0.087, 0.4) | single | 0.73 | 0.25(f) | 2.20 | 0 | 0 | 3.63(f) | |
interior | (0.087, 0.4) | double | 0.95(f) | 0.25(f) | 2.70 | 0.03 | 0.66 | 3.63(f) | |
plasma | (0.190, 0.389) | (0.035, 0.350) | single | 0.960 | 0.880 | 2.70 | 0 | 0 | 4.19 |
plasma | (0.035, 0.350) | single | 0.76 | 0.25(f) | 2.80 | 0 | 0 | 4.19(f) | |
plasma | (0.035, 0.350) | double | 0.95(f) | 0.25(f) | 13 | 0.65 | 0.59 | 4.19(f) |
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Clayton, A.H.A. Spectral Relaxation Imaging Microscopy II: Complex Dynamics. Int. J. Mol. Sci. 2023, 24, 12271. https://doi.org/10.3390/ijms241512271
Clayton AHA. Spectral Relaxation Imaging Microscopy II: Complex Dynamics. International Journal of Molecular Sciences. 2023; 24(15):12271. https://doi.org/10.3390/ijms241512271
Chicago/Turabian StyleClayton, Andrew H. A. 2023. "Spectral Relaxation Imaging Microscopy II: Complex Dynamics" International Journal of Molecular Sciences 24, no. 15: 12271. https://doi.org/10.3390/ijms241512271