Short Culture of Bovine Hepatocytes Biopsied from Dairy Cows as a Model for Toxicological Studies—CYP 1A1 Activity Response to Zearalenone Treatment
Abstract
:1. Introduction
2. Results and Discussion
2.1. The mRNA Expression of Hepatocytes-Specific Genes
2.2. Functional Characterization of Ketotic Hepatocytes after ZEA Treatment
3. Materials and Methods
3.1. Animals
3.2. Liver Biopsies
3.3. Isolation of Hepatocytes
3.4. Cell Culture
3.5. qPCR
3.6. Oil Red Staining
3.7. XTT Cell Viability Assay
3.8. CYP1A1 Activity
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Barć, J.; Flaga, J.; Kozubek, A.; Kowalski, Z.M. Short Culture of Bovine Hepatocytes Biopsied from Dairy Cows as a Model for Toxicological Studies—CYP 1A1 Activity Response to Zearalenone Treatment. Int. J. Mol. Sci. 2023, 24, 12344. https://doi.org/10.3390/ijms241512344
Barć J, Flaga J, Kozubek A, Kowalski ZM. Short Culture of Bovine Hepatocytes Biopsied from Dairy Cows as a Model for Toxicological Studies—CYP 1A1 Activity Response to Zearalenone Treatment. International Journal of Molecular Sciences. 2023; 24(15):12344. https://doi.org/10.3390/ijms241512344
Chicago/Turabian StyleBarć, Justyna, Jadwiga Flaga, Anna Kozubek, and Zygmunt Maciej Kowalski. 2023. "Short Culture of Bovine Hepatocytes Biopsied from Dairy Cows as a Model for Toxicological Studies—CYP 1A1 Activity Response to Zearalenone Treatment" International Journal of Molecular Sciences 24, no. 15: 12344. https://doi.org/10.3390/ijms241512344