Localized Increased Permeability of Blood–Brain Barrier for Antibody Conjugates in the Cuprizone Model of Demyelination
Abstract
:1. Introduction
2. Results
2.1. Validation of the Cuprizone Model of Demyelination in Mice
2.2. Detection of Evans Blue Dye in the Brain of Cuprizone-Treated Mice
2.3. Accumulation of Antibody Conjugates in the Brains of Cuprizone-Treated Mice
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Experimental Design and Modeling of Cuprizone-Induced Demyelination
4.3. rt-PCR Analysis
4.4. Luxol Fast Blue Staining
4.5. Immunohistochemistry of GFAP and VEGFR2
4.6. Magnetic Resonance Imaging (MRI)
4.7. Analysis of Evans Blue Accumulation
4.8. Accumulation of Antibody Conjugates
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Abakumova, T.; Kuzkina, A.; Koshkin, P.; Pozdeeva, D.; Abakumov, M.; Melnikov, P.; Ionova, K.; Gubskii, I.; Gurina, O.; Nukolova, N.; et al. Localized Increased Permeability of Blood–Brain Barrier for Antibody Conjugates in the Cuprizone Model of Demyelination. Int. J. Mol. Sci. 2023, 24, 12688. https://doi.org/10.3390/ijms241612688
Abakumova T, Kuzkina A, Koshkin P, Pozdeeva D, Abakumov M, Melnikov P, Ionova K, Gubskii I, Gurina O, Nukolova N, et al. Localized Increased Permeability of Blood–Brain Barrier for Antibody Conjugates in the Cuprizone Model of Demyelination. International Journal of Molecular Sciences. 2023; 24(16):12688. https://doi.org/10.3390/ijms241612688
Chicago/Turabian StyleAbakumova, Tatiana, Anastasia Kuzkina, Philipp Koshkin, Daria Pozdeeva, Maxim Abakumov, Pavel Melnikov, Klavdia Ionova, Ilia Gubskii, Olga Gurina, Natalia Nukolova, and et al. 2023. "Localized Increased Permeability of Blood–Brain Barrier for Antibody Conjugates in the Cuprizone Model of Demyelination" International Journal of Molecular Sciences 24, no. 16: 12688. https://doi.org/10.3390/ijms241612688