Homeostatic Regulation of the Duox-ROS Defense System: Revelations Based on the Diversity of Gut Bacteria in Silkworms (Bombyx mori)
Abstract
:1. Introduction
2. Result
2.1. Estimation of Gut Microbial Diversity and Abundance
2.2. Intestinal Microbial Composition
2.3. Expression of Genes Related to the Intestinal Duox-ROS Immune System and Determination of Intestinal Antioxidant Enzyme Activity
2.4. Influence of Pathogenic Bacteria on Body Weight and Organismal ROS Levels in the Silkworm
3. Discussion
3.1. Changes in Diet Alter the Composition in the Gut Microbiota of the Traditional Silkworm
3.2. Enriched Intestinal Microbiota Induces Activation of the Duox-ROS Immune Defense Pathway
3.3. Distribution of Key Dominant Genera in the Intestinal Microbiota of the Silkworm
4. Materials and Methods
4.1. Silkworm Strains and Rearing
4.2. Physiological Index Measurements
4.3. Body Weight Statistics and ROS Levels after E. cloacae Treatment
4.4. DNA Preparation, PCR, Pyrophosphate Sequencing and Sequence Processing
4.5. Real-Time Quantitative Polymerase Chain Reaction
4.6. Sequencing Data Processing
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shu, Q.; Guo, X.; Tian, C.; Wang, Y.; Zhang, X.; Cheng, J.; Li, F.; Li, B. Homeostatic Regulation of the Duox-ROS Defense System: Revelations Based on the Diversity of Gut Bacteria in Silkworms (Bombyx mori). Int. J. Mol. Sci. 2023, 24, 12731. https://doi.org/10.3390/ijms241612731
Shu Q, Guo X, Tian C, Wang Y, Zhang X, Cheng J, Li F, Li B. Homeostatic Regulation of the Duox-ROS Defense System: Revelations Based on the Diversity of Gut Bacteria in Silkworms (Bombyx mori). International Journal of Molecular Sciences. 2023; 24(16):12731. https://doi.org/10.3390/ijms241612731
Chicago/Turabian StyleShu, Qilong, Xiqian Guo, Chao Tian, Yuanfei Wang, Xiaoxia Zhang, Jialu Cheng, Fanchi Li, and Bing Li. 2023. "Homeostatic Regulation of the Duox-ROS Defense System: Revelations Based on the Diversity of Gut Bacteria in Silkworms (Bombyx mori)" International Journal of Molecular Sciences 24, no. 16: 12731. https://doi.org/10.3390/ijms241612731