Comprehensive Analysis of Ferroptosis Regulators with Regard to PD-L1 and Immune Infiltration in Low-Grade Glioma
Abstract
:1. Introduction
2. Results
2.1. Expression Divergence of FRGs between LGG and Normal Tissues
2.2. Consensus Clustering Analysis of FRGs Revealed Significant Differences in Baseline Characteristics and Survival between Two Patient Clusters
2.3. Association of FRGs with CD274 Expression Level and Immune Cell Infiltration in LGG
2.4. Four Key FRGs (LPCAT3, SLC1A5, HSPA5, and NFE2L2) Were Upregulated in Glioma Tissues
2.5. Correlation between Four Key FRGs and the TME Heterogeneity
2.6. Construction of the Prognostic Model of Four Key FRGs in LGG
2.7. Correlation between Four Prognostic FRGs’ Expression Levels and Immune Infiltration Levels in LGG
2.8. Construction of an mRNA–miRNA–lncRNA Network
3. Discussion
4. Materials and Methods
4.1. Data Acquisition
4.2. Subgroup Analysis
4.3. Co-Expression Analysis
4.4. Immune Score Analysis
4.5. Survival Analysis
4.6. Competing Endogenous RNA Network Construction
4.7. Cell Culture
4.8. Plasmids and Transfection
4.9. Quantitative Real-Time PCR
4.10. Quantitation of IHC Images
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Feature | Cluster 1 | Cluster 2 | p-Value | |
---|---|---|---|---|
Status | Alive | 344 | 44 | <0.0001 |
Dead | 78 | 47 | ||
Age | Mean (SD) | 41.3 (12.7) | 50.7 (13.7) | <0.0001 |
Median [Min Max] | 39 [14 74] | 53 [24 87] | ||
Gender | Female | 189 | 39 | 0.826 |
Male | 233 | 52 | ||
Race | Asian | 6 | 2 | 0.145 |
Black | 14 | 7 | ||
White | 392 | 81 | ||
American Indian | 1 | |||
Grade | Discrepancy | 1 | <0.0001 | |
G2 | 234 | 15 | ||
G3 | 187 | 76 | ||
Radiation therapy | Non-radiation | 113 | 8 | <0.0001 |
Radiation | 101 | 41 |
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Luan, Y.; Chen, Y.; Wang, Y.; Shu, M. Comprehensive Analysis of Ferroptosis Regulators with Regard to PD-L1 and Immune Infiltration in Low-Grade Glioma. Int. J. Mol. Sci. 2023, 24, 12880. https://doi.org/10.3390/ijms241612880
Luan Y, Chen Y, Wang Y, Shu M. Comprehensive Analysis of Ferroptosis Regulators with Regard to PD-L1 and Immune Infiltration in Low-Grade Glioma. International Journal of Molecular Sciences. 2023; 24(16):12880. https://doi.org/10.3390/ijms241612880
Chicago/Turabian StyleLuan, Yuxuan, Yuling Chen, Yilin Wang, and Minfeng Shu. 2023. "Comprehensive Analysis of Ferroptosis Regulators with Regard to PD-L1 and Immune Infiltration in Low-Grade Glioma" International Journal of Molecular Sciences 24, no. 16: 12880. https://doi.org/10.3390/ijms241612880