Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
Abstract
:1. Introduction
2. Results
2.1. Effects of CsA on Cold Thermoreceptors’ Nerve Activity
2.2. Effects of CsA on the Shape of NTIs of Cold Thermoreceptors
2.3. Effect of CsA on Blinking and Tearing
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Surgical Procedure
4.3. Cyclosporine A
4.4. Recording of the Electrical Activity of Corneal Cold Thermosensitive Nerve Terminals
4.5. Electrophysiological Recording Analysis
4.6. Blinking Frequency and Tearing Rate in Awake Guinea Pigs
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Control Corneas (n = 7) | Tear-Deficient Corneas (n = 7) | |||
---|---|---|---|---|
Before | During CsA | Before | During CsA | |
Spontaneous activity at 34 °C (imps/s) | 6.4 ± 0.9 | 6.4 ± 0.8 | 10.2 ± 0.3 | 11.4 ± 1.1 |
Cooling threshold (°C) | 32.3 ± 0.4 | 31.7 ± 0.5 | 32.4 ± 0.2 | 32.4 ± 0.4 |
Peak response to cold (imp/s) | 28.4 ± 5.4 | 21.6 ± 3.5 | 32.7 ± 4.5 | 23 ± 4.2 * |
Parameters of NTI Shape | Control Corneas (n = 7) | Tear-Deficient Corneas (n = 3) | ||
---|---|---|---|---|
Before | During CsA | Before | During CsA | |
+peak (µV) | 20.97 ± 2.54 | 25.62 ± 4.28 | 16.72 ± 1.47 | 15.49 ± 0.59 |
−peak (µV) | 21.57 ± 2.63 | 26.10 ± 3.86 | 17.57 ± 1.86 | 16.56 ± 0.37 |
Duration (ms) | 2.75 ± 0.14 | 2.85 ± 0.20 | 2.13 ± 0.12 * | 2.27 ± 0.19 |
+dV/dt max normalized (µV/ms) | 1853 ± 227 | 1766 ± 257 | 2033 ± 251 | 1788 ± 1.7 |
−dV/dt max normalized (µV/ms) | 2207 ± 202 | 2222 ± 191 | 2472 ± 233 | 2512 ± 190 |
Control Eyes (n = 24) | Tear-Deficient Eyes (n = 6) | |||
---|---|---|---|---|
Before | During CsA | Before | During CsA | |
Blinking frequency (blinks/min) | 1.7 ± 0.4 | 21.0 ± 1.6 ** | 10 ± 3.7 | 14.7 ± 2.3 |
Tearing rate (mm) | 12.8 ± 1.2 | 24.0 ± 1.6 ** | 4.0 ± 1.0 | 7.8 ± 2.3 |
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Gyenes, A.; Tapasztó, Z.; Quirce, S.; Luna, C.; Frutos-Rincón, L.; Gallar, J.; Acosta, M.C.; Kovács, I. Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals. Int. J. Mol. Sci. 2023, 24, 13025. https://doi.org/10.3390/ijms241613025
Gyenes A, Tapasztó Z, Quirce S, Luna C, Frutos-Rincón L, Gallar J, Acosta MC, Kovács I. Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals. International Journal of Molecular Sciences. 2023; 24(16):13025. https://doi.org/10.3390/ijms241613025
Chicago/Turabian StyleGyenes, Andrea, Zsófia Tapasztó, Susana Quirce, Carolina Luna, Laura Frutos-Rincón, Juana Gallar, M. Carmen Acosta, and Illés Kovács. 2023. "Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals" International Journal of Molecular Sciences 24, no. 16: 13025. https://doi.org/10.3390/ijms241613025