Long-Lasting Epigenetic Changes in the Dopamine Transporter in Adult Animals Exposed to Amphetamine during Embryogenesis: Investigating Behavioral Effects
Abstract
:1. Introduction
2. Results
2.1. Chronic Exposure to Amphetamine during Embryogenesis Induces Amphetamine Hypersensitivity in Adult Animals
2.2. Chronic Exposure to Amphetamine during Embryogenesis Alters Histone Marks in the dat-1 Gene and Decreases Expression of DAT-1 in Adult Animals
2.3. The Long-Term Effects Caused by Amphetamine during Embryogenesis in Adult Animals Are Blocked by Genetic or Pharmacological Inhibition of Specific Lysine 9 Methyltransferases
3. Discussion
4. Materials and Methods
4.1. Worm Husbandry and Strains
4.2. Embryonic Exposure to Amphetamine
4.3. Swimming-Induced Paralysis (SWIP) Assays
4.4. Chromatin Immunoprecipitation (ChIP) Assays
4.5. Western Blot
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ke, T.; Ambigapathy, G.; Ton, T.; Dhasarathy, A.; Carvelli, L. Long-Lasting Epigenetic Changes in the Dopamine Transporter in Adult Animals Exposed to Amphetamine during Embryogenesis: Investigating Behavioral Effects. Int. J. Mol. Sci. 2023, 24, 13092. https://doi.org/10.3390/ijms241713092
Ke T, Ambigapathy G, Ton T, Dhasarathy A, Carvelli L. Long-Lasting Epigenetic Changes in the Dopamine Transporter in Adult Animals Exposed to Amphetamine during Embryogenesis: Investigating Behavioral Effects. International Journal of Molecular Sciences. 2023; 24(17):13092. https://doi.org/10.3390/ijms241713092
Chicago/Turabian StyleKe, Tao, Ganesh Ambigapathy, Thanh Ton, Archana Dhasarathy, and Lucia Carvelli. 2023. "Long-Lasting Epigenetic Changes in the Dopamine Transporter in Adult Animals Exposed to Amphetamine during Embryogenesis: Investigating Behavioral Effects" International Journal of Molecular Sciences 24, no. 17: 13092. https://doi.org/10.3390/ijms241713092