Impact of Classic Adrenal Secretagogues on mRNA Levels of Urotensin II and Its Receptor in Adrenal Gland of Rats
Abstract
:1. Introduction
2. Results
2.1. In Vitro Experiments
2.2. In Vivo Experiments
3. Discussion
4. Materials and Methods
4.1. Animals and Reagents
4.2. In Vitro Experiments
4.2.1. Primary Adrenocortical Cell Culture
4.2.2. Freshly Isolated Rat Adrenal Cells
4.2.3. RNA Extraction
4.2.4. Reverse Transcription and qPCR
4.2.5. Microarray Expression Analysis
4.2.6. Corticosterone, Aldosterone, and Urotensin II Detection
4.3. In Vivo Experiments
4.3.1. The Effects of Acute and Prolonged ACTH Injections on Uts2 and Uts2r mRNA Levels in Rat Adrenal Glands Were Determined
4.3.2. Regeneration Model
4.3.3. Unilateral Adrenalectomy
4.3.4. Effects of Gonadectomy and Sex Hormones’ Supplementation on Uts2 and Uts2r Gene Expressions
4.4. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jopek, K.; Tyczewska, M.; Szyszka, M.; Blatkiewicz, M.; Jopek, M.; Malendowicz, L.K.; Ruciński, M. Impact of Classic Adrenal Secretagogues on mRNA Levels of Urotensin II and Its Receptor in Adrenal Gland of Rats. Int. J. Mol. Sci. 2023, 24, 13412. https://doi.org/10.3390/ijms241713412
Jopek K, Tyczewska M, Szyszka M, Blatkiewicz M, Jopek M, Malendowicz LK, Ruciński M. Impact of Classic Adrenal Secretagogues on mRNA Levels of Urotensin II and Its Receptor in Adrenal Gland of Rats. International Journal of Molecular Sciences. 2023; 24(17):13412. https://doi.org/10.3390/ijms241713412
Chicago/Turabian StyleJopek, Karol, Marianna Tyczewska, Marta Szyszka, Małgorzata Blatkiewicz, Maria Jopek, Ludwik K. Malendowicz, and Marcin Ruciński. 2023. "Impact of Classic Adrenal Secretagogues on mRNA Levels of Urotensin II and Its Receptor in Adrenal Gland of Rats" International Journal of Molecular Sciences 24, no. 17: 13412. https://doi.org/10.3390/ijms241713412