Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of the Heterocellular Bone Marrow Population in Primary Culture
2.2. Stromal Lineage Cells and Extracellular Matrix
2.3. Transcriptomic Analysis of Genes Encoding Regulatory Molecules
3. Materials and Methods
3.1. Experimental Animals
3.2. Bone Marrow Cell Isolation and Enumeration
3.3. Cell Culture
3.4. In Vitro Simulation of Microgravity Effects (3D Clinorotation)
3.5. Histochemical Evaluation of ECM Components
3.6. Immunophenotyping of Cultured Primary Bone Marrow Cells
3.7. Quantitative Real-Time PCR
3.8. Paracrine Activity
3.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Downregulation | Upregulation |
---|---|
Cell adhesion | Negative regulation of cell-matrix adhesion |
Lama2, Lamb2, Tgfbi, Col3a1, Sparc | Thbs1 |
Cell differentiation | Negative regulation of cell adhesion |
Lama2, Lamb2, Tgfbi, Col2a1, Col3a1, Mmp8, Mmp9, Lama2, Lamb2, Spp1, Timp2 | Thbs1, Mmp2 |
Positive regulation of cell communication | Negative regulation of angiogenesis |
Lama2, Col3a1, Spp1, Timp2, Timp3, Mmp8, Mmp9 | Adamts1, Adamts5, Col4a2, Thbs1, Emilin1 |
Regulation of cell migration | Cell migration |
Lama2, Col3a1, Sparc, Mmp9, Timp1 | Mmp2, Lama3, Thbs1, Emilin1 |
Extracellular matrix organization | Extracellular matrix disassembly |
Tgfbi, Col2a1, Col3a1, Mmp8, Mmp9, Lamb2 | Mmp13 |
Negative regulation of metallopeptidase activity | Collagen catabolic process |
Timp1, Timp2, Timp3 | Adamts2, Mmp1a, Mmp2, Mmp13 |
Basement membrane organization | |
Col4a1, Col4a2, Lama3 |
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Markina, E.; Tyrina, E.; Ratushnyy, A.; Andreeva, E.; Buravkova, L. Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions. Int. J. Mol. Sci. 2023, 24, 13746. https://doi.org/10.3390/ijms241813746
Markina E, Tyrina E, Ratushnyy A, Andreeva E, Buravkova L. Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions. International Journal of Molecular Sciences. 2023; 24(18):13746. https://doi.org/10.3390/ijms241813746
Chicago/Turabian StyleMarkina, Elena, Ekaterina Tyrina, Andrey Ratushnyy, Elena Andreeva, and Ludmila Buravkova. 2023. "Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions" International Journal of Molecular Sciences 24, no. 18: 13746. https://doi.org/10.3390/ijms241813746