A Novel Lipid Metabolism and Endoplasmic Reticulum Stress-Related Risk Model for Predicting Immune Infiltration and Prognosis in Colorectal Cancer
Abstract
:1. Introduction
2. Results
2.1. Exploration of Differentially Expressed LERGs in CRC
2.2. Construction of the Prognostic Model
2.3. Validation of the Prognostic Model across Multiple External Datasets
2.4. Incidence Risk and Independent Prognostic Analysis
2.5. Correlation Analysis of the Disease Risk Score with Multiple Clinical Indicators
2.6. The Mutational Landscape for CRC and Tumor Mutational Burden (TMB)
2.7. Drug Sensitivity and Molecular Pathway for the Risk Model
2.8. Relationship between the Risk Model and the Immune Microenvironment
2.9. Construction of a WGCNA Co-Expression Network and Upstream Mechanism Analysis of Hub Genes
2.10. Validation of Hub Genes in the CRC Clinical Sample
2.11. Single-Cell Analysis for the Immune Landscape and Gene Expression in CRC
3. Discussion
4. Materials and Methods
4.1. Data Sources and Collection
4.2. Construction and Validation of a Risk Model
4.3. Construction of a Predictive Nomogram
4.4. TMB Analysis
4.5. Drug Sensitivity Analysis
4.6. Gene Set Variation Analysis (GSVA) and Enrichment Analysis (GSEA)
4.7. Immune Infiltration Analysis
4.8. Weighted Gene Co-Expression Network Analysis (WGCNA)
4.9. Construction of a PPI Network and Prediction of the Transcription Factor (TF)
4.10. Clinical Samples’ Verification of Hub Genes
4.11. Single-Cell RNA Sequencing Dataset Analysis
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jin, H.; Xia, B.; Wang, J.; Qi, S.; Jing, W.; Deng, K.; Yang, J. A Novel Lipid Metabolism and Endoplasmic Reticulum Stress-Related Risk Model for Predicting Immune Infiltration and Prognosis in Colorectal Cancer. Int. J. Mol. Sci. 2023, 24, 13854. https://doi.org/10.3390/ijms241813854
Jin H, Xia B, Wang J, Qi S, Jing W, Deng K, Yang J. A Novel Lipid Metabolism and Endoplasmic Reticulum Stress-Related Risk Model for Predicting Immune Infiltration and Prognosis in Colorectal Cancer. International Journal of Molecular Sciences. 2023; 24(18):13854. https://doi.org/10.3390/ijms241813854
Chicago/Turabian StyleJin, Haoran, Bihan Xia, Jin Wang, Shaochong Qi, Weina Jing, Kai Deng, and Jinlin Yang. 2023. "A Novel Lipid Metabolism and Endoplasmic Reticulum Stress-Related Risk Model for Predicting Immune Infiltration and Prognosis in Colorectal Cancer" International Journal of Molecular Sciences 24, no. 18: 13854. https://doi.org/10.3390/ijms241813854