Long Noncoding RNA ZBED5-AS1 Facilitates Tumor Progression and Metastasis in Lung Adenocarcinoma via ZNF146/ATR/Chk1 Axis
Abstract
:1. Introduction
2. Results
2.1. ZBED5-AS1 Is Highly Expressed in LUAD
2.2. ZBED5-AS1 Promotes Malignant Phenotypes of LUAD Cells
2.3. ZNF146 Enhances the Malignant Phenotypes of LUAD Cells
2.4. ZNF146 Knockdown Inhibits LUAD Cell Proliferation and Invasion Mediated by ZBED5-AS1
2.5. ZBED5-AS1 Silencing Suppresses the Activation of ATR/Chk1 Signaling Pathway
2.6. Si-ZBED5-AS1 Exosomes Inhibite LUAD Cells Proliferation and Metastasis
3. Discussion
4. Materials and Methods
4.1. Patient Samples
4.2. Animal Experiments
4.3. Cell Culture
4.4. Cell Transfection
4.5. Subcellular Fractionation
4.6. Exosome Isolation and Identification
4.7. RNA Extraction and RT-qPCR
4.8. Exosome Treatment
4.9. Cell Proliferation Assay
4.10. Transwell Assay
4.11. Flow Cytometry
4.12. Western Blot
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Characteristics | Low Expression | High Expression | p Value |
---|---|---|---|
n | 28 | 28 | |
gender, n (%) | 1.0000 | ||
female | 15 (26.8%) | 15 (26.8%) | |
male | 13 (23.2%) | 13 (23.2%) | |
age, n (%) | 0.5842 | ||
≤65 | 18 (32.1%) | 16 (28.6%) | |
>65 | 10 (17.9%) | 12 (21.4%) | |
T, n (%) | 0.3881 | ||
T1 | 22 (39.3%) | 21 (37.5%) | |
T2 | 4 (7.1%) | 5 (8.9%) | |
T4 | 2 (3.6%) | 0 (0%) | |
T3 | 0 (0%) | 1 (1.8%) | |
X | 0 (0%) | 1 (1.8%) | |
N.stage, n (%) | 0.0860 | ||
N0 | 22 (39.3%) | 26 (46.4%) | |
N1 | 6 (10.7%) | 1 (1.8%) | |
X | 0 (0%) | 1 (1.8%) | |
M.stage, n (%) | 0.9999 | ||
M0 | 27 (48.2%) | 27 (48.2%) | |
M1 | 1 (1.8%) | 0 (0%) | |
X | 0 (0%) | 1 (1.8%) | |
Tumor size, n (%) | 0.1764 | ||
>2 | 9 (16.1%) | 14 (25%) | |
≤2 | 19 (33.9%) | 13 (23.2%) | |
X | 0 (0%) | 1 (1.8%) | |
CEA (μg/L), median (IQR) | 2.4 (1.35, 4.75) | 2.45 (1.9, 6.3) | 0.4775 |
NSE (ng/mL), median (IQR) | 12.6 (9.95, 19.45) | 13.1 (11, 16.2) | 0.6602 |
ProGRP (ng/L), mean ± sd | 48.708 ± 14.977 | 51.05 ± 23.442 | 0.7021 |
D-dimer, median (IQR) | 0.345 (0.3, 0.72) | 0.91 (0.37, 1.06) | 0.0245 |
LDH (U/L), median (IQR) | 178 (162, 189.5) | 187 (178.5, 236) | 0.0480 |
PLT counts, median (IQR) | 231.5 (185.5, 276.5) | 253 (222.5, 294) | 0.2158 |
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Jiang, F.; Huang, X.; Ling, L.; Tang, S.; Zhou, H.; Cai, X.; Wang, Y. Long Noncoding RNA ZBED5-AS1 Facilitates Tumor Progression and Metastasis in Lung Adenocarcinoma via ZNF146/ATR/Chk1 Axis. Int. J. Mol. Sci. 2023, 24, 13925. https://doi.org/10.3390/ijms241813925
Jiang F, Huang X, Ling L, Tang S, Zhou H, Cai X, Wang Y. Long Noncoding RNA ZBED5-AS1 Facilitates Tumor Progression and Metastasis in Lung Adenocarcinoma via ZNF146/ATR/Chk1 Axis. International Journal of Molecular Sciences. 2023; 24(18):13925. https://doi.org/10.3390/ijms241813925
Chicago/Turabian StyleJiang, Feng, Xiaolu Huang, Liqun Ling, Shiyi Tang, Huixin Zhou, Xueding Cai, and Yumin Wang. 2023. "Long Noncoding RNA ZBED5-AS1 Facilitates Tumor Progression and Metastasis in Lung Adenocarcinoma via ZNF146/ATR/Chk1 Axis" International Journal of Molecular Sciences 24, no. 18: 13925. https://doi.org/10.3390/ijms241813925