Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence
Abstract
:1. Introduction
2. Results
2.1. Ginsenoside F1 Preserved the Telomere Length in Aged Somatic Cells
2.2. Ginsenoside F1 Modulated the Protein Kinase B (AKT) and Mitogen-Activated Protein Kinase (MAPK) Pathways in Aged Somatic Cells
2.3. Ginsenoside F1 Ameliorated Mitochondrial Dysfunction and Cellular Senescence in Aged Somatic Cells
2.4. TRF2 Depletion-Induced Cellular Senescence
2.5. Molecular Docking Simulation in F1 and TRF2
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cells and Cell Culture
4.3. Cell Aging
4.4. Telomere Length Assay
4.5. PCR Array
4.6. Antibody Array
4.7. Western Blot
4.8. Intracellular Superoxide Detection
4.9. ATP Assay
4.10. Senescence-Associated β-Galactosidase (SA-βG) Staining
4.11. Real-Time qPCR
4.12. Immunofluorescence
4.13. Molecular Docking
4.14. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Hou, J.; Yun, Y.; Jeon, B.; Baek, J.; Kim, S. Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence. Int. J. Mol. Sci. 2023, 24, 14241. https://doi.org/10.3390/ijms241814241
Hou J, Yun Y, Jeon B, Baek J, Kim S. Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence. International Journal of Molecular Sciences. 2023; 24(18):14241. https://doi.org/10.3390/ijms241814241
Chicago/Turabian StyleHou, Jingang, Yeejin Yun, Byeongmin Jeon, Jongin Baek, and Sunchang Kim. 2023. "Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence" International Journal of Molecular Sciences 24, no. 18: 14241. https://doi.org/10.3390/ijms241814241