Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments
Abstract
:1. Introduction
2. Results
2.1. Curating the Proteome of Different Arabidopsis Spaceflight Experiments
2.2. Principal Component Analysis
2.3. The Direct Intersection of the Four Proteomics Datasets
2.4. Comparing BRIC PDFU and BRIC LED Datasets: Impact of Light Conditions
2.5. Eliminating Microgravity from the Comparison
3. Discussion
3.1. The Determinant of the Arabidopsis Proteomic Response to the Spaceflight Environment Is Multidimensional
3.2. Protein Intersections and Functional Implications
3.3. Integration of Light Signals in Spaceflight Leads to Altered Metabolism
3.4. Disentangling Microgravity from Other Plant Spaceflight Responses
4. Materials and Methods
Accessing and Analyzing Individual Proteomics Datasets
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Symbol | Name | ABRS | EMCS | BRIC PDFU | BRIC LED |
---|---|---|---|---|---|---|
Proteins shared by all the datasets | Log 2 Fold Change (L2FC) | |||||
AT1G27090 | Glycine-rich protein | −0.66 | 0.29 | 0.23 | −0.37 | |
AT1G72150 | PATL1 | Patellin-1 | 0.71 | −1.318 | −0.17 | 0.57 |
AT4G20260 | PCAP1 | Plasma-membrane-associated cation-binding protein 1 | 0.73 | −0.79 | −0.44 | 0.49 |
AT4G33010 | GLDP1 | Glycine decarboxylase P-protein 1 | −0.96 | −1.27 | −0.35 | −0.47 |
AT5G26260 | Leucine-rich repeat receptor-like kinase1 | 0.59 | 0.56 | −0.414 | 0.8 | |
Proteins shared by EMCS, BRIC PDFU, and BRIC LED but not ABRS | ||||||
AT1G74470 | CHLP | Geranylgeranyl diphosphate reductase | 0.37 | 0.54 | −0.54 | |
AT3G16470 | JAL35 | Jacalin-related lectin 35 | −1.53 | −0.46 | −0.35 | |
AT3G22640 | PAP85 | Vicilin-like seed storage protein | −0.901 | 0.02 | 3.31 | |
AT5G19550 | ASP2 | Aspartate aminotransferase | 1.13 | −0.14 | 0.31 | |
AT5G37600 | GLN1-1 | Glutamine synthetase cytosolic isozyme 1-1 | 0.82 | −0.06 | 0.23 |
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Olanrewaju, G.O.; Kruse, C.P.S.; Wyatt, S.E. Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments. Int. J. Mol. Sci. 2023, 24, 14425. https://doi.org/10.3390/ijms241914425
Olanrewaju GO, Kruse CPS, Wyatt SE. Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments. International Journal of Molecular Sciences. 2023; 24(19):14425. https://doi.org/10.3390/ijms241914425
Chicago/Turabian StyleOlanrewaju, Gbolaga O., Colin P. S. Kruse, and Sarah E. Wyatt. 2023. "Functional Meta-Analysis of the Proteomic Responses of Arabidopsis Seedlings to the Spaceflight Environment Reveals Multi-Dimensional Sources of Variability across Spaceflight Experiments" International Journal of Molecular Sciences 24, no. 19: 14425. https://doi.org/10.3390/ijms241914425