Identification of Genomic Regions Implicated in Susceptibility to Schistosoma mansoni Infection in a Murine Backcross Genetic Model
Abstract
:1. Introduction
2. Results
2.1. There Was a Different Susceptibility to Schistosomiasis in Mice Related to the Genetic Background but Not to Mouse Sex
2.2. Parasitological, Pathological, and White Blood Cell Populations Correlated in the BX Cohort
2.3. Identification of Different Degrees of Schistosomiasis Disease in the BX Mouse Cohort
2.4. Pathophenotypic Variation in the BX Cohort in the Groups Identified
2.5. Identifying Chromosomal Regions Associated with Susceptibility
2.6. Identification of Genomic Regions Linked to Disease Susceptibility across the Four Groups Identified in the BX
3. Discussion
4. Materials and Methods
4.1. Animals and Parasites
4.2. BX Mouse Cohort Infection and Parasitological and Pathological Traits Obtained
4.3. Peripheral and Spleen White Blood Cell Subpopulations Quantified via Flow Cytometry
4.4. Detection of Anti-S. mansoni Antibodies in Mice Infected with the Parasite Using ELISA
4.5. SNP Genotyping, Quality Control, and Linkage Analysis
4.6. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hernández-Goenaga, J.; López-Abán, J.; Blanco-Gómez, A.; Vicente, B.; Burguillo, F.J.; Pérez-Losada, J.; Muro, A. Identification of Genomic Regions Implicated in Susceptibility to Schistosoma mansoni Infection in a Murine Backcross Genetic Model. Int. J. Mol. Sci. 2023, 24, 14768. https://doi.org/10.3390/ijms241914768
Hernández-Goenaga J, López-Abán J, Blanco-Gómez A, Vicente B, Burguillo FJ, Pérez-Losada J, Muro A. Identification of Genomic Regions Implicated in Susceptibility to Schistosoma mansoni Infection in a Murine Backcross Genetic Model. International Journal of Molecular Sciences. 2023; 24(19):14768. https://doi.org/10.3390/ijms241914768
Chicago/Turabian StyleHernández-Goenaga, Juan, Julio López-Abán, Adrián Blanco-Gómez, Belén Vicente, Francisco Javier Burguillo, Jesús Pérez-Losada, and Antonio Muro. 2023. "Identification of Genomic Regions Implicated in Susceptibility to Schistosoma mansoni Infection in a Murine Backcross Genetic Model" International Journal of Molecular Sciences 24, no. 19: 14768. https://doi.org/10.3390/ijms241914768