Identification of Potential Mechanisms of Rk1 Combination with Rg5 in the Treatment of Type II Diabetes Mellitus by Integrating Network Pharmacology and Experimental Validation
Abstract
:1. Introduction
2. Results
2.1. Network Pharmacology Prediction
2.2. Effect of Rk1+Rg5 Treatment in db/db Mice
2.3. Effect of Rk1+Rg5 on the Glucose Metabolism in db/db Mice
2.4. Effects of Rk1+Rg5 on Glucose Transporter in the Skeletal Muscle of db/db Mice
2.5. Effects of Rk1+Rg5 on Glucose Uptake in L6 Cells
2.6. Effect of Rk1+Rg5 on the Potential Mechanism in T2DM
3. Discussion
4. Materials and Methods
4.1. Materials and Chemicals
4.2. Potential Target Proteins, PPI, GO and KEGG Pathway Enrichment Analysis
4.3. Molecular Docking
4.4. Animals and Experimental Design
4.5. Analysis of FBG, OGTT, and ITT
4.6. Biochemical Analyses
4.7. HOMA-IR and Index of HOMA-β Analysis
4.8. Histopathological Analysis
4.9. Immunofluorescence of Skeletal Muscle
4.10. Cell Culture and Differentiation
4.11. PA-Induced Insulin Resistance Model in L6 Cells
4.12. Glucose Consumption
4.13. Western Blotting Analysis
4.14. RNA Sequence Analysis
4.15. Data Processing and Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Receptor | Binding Interaction Energy (kcal/mol) | VDW Interaction Energy (kcal/mol) | Electrostatic Interaction Energy (kcal/mol) | |||
---|---|---|---|---|---|---|
Rk1 | Rg5 | Rk1 | Rg5 | Rk1 | Rg5 | |
PI3KR1 | −1.64 | −1.06 | −6.89 | PI3KR1 | −1.64 | −1.06 |
SRC | −4.24 | −5.06 | −9.12 | SRC | −4.24 | −5.06 |
PIK3CA | −4.15 | −3.65 | −9.28 | PIK3CA | −4.15 | −3.65 |
STAT3 | −0.99 | −1.71 | −6.29 | STAT3 | −0.99 | −1.71 |
GRB2 | −5.62 | −5.81 | −10.71 | GRB2 | −5.62 | −5.81 |
HRAS | −5.13 | −6.19 | −10.11 | HRAS | −5.13 | −6.19 |
PTPN11 | −4.31 | −5.14 | −9.6 | PTPN11 | −4.31 | −5.14 |
MAPK1 | −5.42 | −4.52 | −10.49 | MAPK1 | −5.42 | −4.52 |
HSP90AA1 | −4.86 | −2.84 | −9.96 | HSP90AA1 | −4.86 | −2.84 |
Akt1 | −4.95 | −5.46 | −9.68 | Akt1 | −4.95 | −5.46 |
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Liu, Y.; Zhang, J.; An, C.; Liu, C.; Zhang, Q.; Ding, H.; Ma, S.; Xue, W. Identification of Potential Mechanisms of Rk1 Combination with Rg5 in the Treatment of Type II Diabetes Mellitus by Integrating Network Pharmacology and Experimental Validation. Int. J. Mol. Sci. 2023, 24, 14828. https://doi.org/10.3390/ijms241914828
Liu Y, Zhang J, An C, Liu C, Zhang Q, Ding H, Ma S, Xue W. Identification of Potential Mechanisms of Rk1 Combination with Rg5 in the Treatment of Type II Diabetes Mellitus by Integrating Network Pharmacology and Experimental Validation. International Journal of Molecular Sciences. 2023; 24(19):14828. https://doi.org/10.3390/ijms241914828
Chicago/Turabian StyleLiu, Yao, Jingjing Zhang, Chao An, Chen Liu, Qiwen Zhang, Hao Ding, Saijian Ma, and Wenjiao Xue. 2023. "Identification of Potential Mechanisms of Rk1 Combination with Rg5 in the Treatment of Type II Diabetes Mellitus by Integrating Network Pharmacology and Experimental Validation" International Journal of Molecular Sciences 24, no. 19: 14828. https://doi.org/10.3390/ijms241914828